Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses

Svetlana Pavlukhina, Iryna Zhuk, Almagul Mentbayeva, Emily Rautenberg, Wei Chang, Xiaojun Yu, Betsy Van De Belt-Gritter, Henk J. Busscher, Henny C. Van Der Mei, Svetlana A. Sukhishvili

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    We report pH/bacteria-responsive nanocomposite coatings with multiple mechanisms of antibacterial protection that include the permanent retention of antimicrobials, bacteria-triggered release of antibiotics and bacteria-induced film swelling. A novel small-molecule-hosting film was constructed using layer-by-layer deposition of montmorillonite (MMT) clay nanoplatelets and polyacrylic acid (PAA) components, both of which carry a negative charge at neutral pH. The films were highly swollen in water, and they exhibited major changes in swelling as a function of pH. Under physiologic conditions (pH 7.5, 0.2 M NaCl), hydrogel-like MMT/PAA films took up and sequestered ~45% of the dry film matrix mass of the antibiotic gentamicin, causing dramatic film deswelling. Gentamicin remained sequestrated within the films for months under physiologic conditions and therefore did not contribute to the development of antibiotic resistance. When challenged with bacteria (Staphylococcus aureus, Staphylococcus epidermidis or Escherichia coli), the coatings released PAA-bound gentamicin because of bacteria-induced acidification of the immediate environment, whereas gentamicin adsorbed to MMT nanoplatelets remained bound within the coating, affording sustained antibacterial protection. Moreover, an increase in film swelling after gentamicin release further hindered bacterial adhesion. These multiple bacteria-triggered responses, together with nontoxicity to tissue cells, make these coatings promising candidates for protecting biomaterial implants and devices against bacterial colonization.

    Original languageEnglish
    Article numbere121
    JournalNPG Asia Materials
    Volume6
    Issue number8
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Nanocomposite films
    Nanocomposites
    Bacteria
    bacteria
    carbopol 940
    nanocomposites
    Molecules
    Gentamicins
    Bentonite
    Coating
    antibiotics
    Swelling
    molecules
    Antibiotics
    montmorillonite
    Clay minerals
    swelling
    coatings
    Coatings
    staphylococcus

    ASJC Scopus subject areas

    • Materials Science(all)
    • Condensed Matter Physics
    • Modelling and Simulation

    Cite this

    Pavlukhina, S., Zhuk, I., Mentbayeva, A., Rautenberg, E., Chang, W., Yu, X., ... Sukhishvili, S. A. (2014). Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses. NPG Asia Materials, 6(8), [e121]. https://doi.org/10.1038/am.2014.63

    Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses. / Pavlukhina, Svetlana; Zhuk, Iryna; Mentbayeva, Almagul; Rautenberg, Emily; Chang, Wei; Yu, Xiaojun; Van De Belt-Gritter, Betsy; Busscher, Henk J.; Van Der Mei, Henny C.; Sukhishvili, Svetlana A.

    In: NPG Asia Materials, Vol. 6, No. 8, e121, 2014.

    Research output: Contribution to journalArticle

    Pavlukhina, S, Zhuk, I, Mentbayeva, A, Rautenberg, E, Chang, W, Yu, X, Van De Belt-Gritter, B, Busscher, HJ, Van Der Mei, HC & Sukhishvili, SA 2014, 'Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses', NPG Asia Materials, vol. 6, no. 8, e121. https://doi.org/10.1038/am.2014.63
    Pavlukhina, Svetlana ; Zhuk, Iryna ; Mentbayeva, Almagul ; Rautenberg, Emily ; Chang, Wei ; Yu, Xiaojun ; Van De Belt-Gritter, Betsy ; Busscher, Henk J. ; Van Der Mei, Henny C. ; Sukhishvili, Svetlana A. / Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses. In: NPG Asia Materials. 2014 ; Vol. 6, No. 8.
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