Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation

N. Henriette Uhlenhaut, Susanne Jakob, Katrin Anlag, Tobias Eisenberger, Ryohei Sekido, Jana Kress, Anna Corina Treier, Claudia Klugmann, Christian Klasen, Nadine I. Holter, Dieter Riethmacher, Günther Schütz, Austin J. Cooney, Robin Lovell-Badge, Mathias Treier

Research output: Contribution to journalArticle

488 Citations (Scopus)

Abstract

In mammals, the transcription factor SRY, encoded by the Y chromosome, is normally responsible for triggering the indifferent gonads to develop as testes rather than ovaries. However, testis differentiation can occur in its absence. Here we demonstrate in the mouse that a single factor, the forkhead transcriptional regulator FOXL2, is required to prevent transdifferentiation of an adult ovary to a testis. Inducible deletion of Foxl2 in adult ovarian follicles leads to immediate upregulation of testis-specific genes including the critical SRY target gene Sox9. Concordantly, reprogramming of granulosa and theca cell lineages into Sertoli-like and Leydig-like cell lineages occurs with testosterone levels comparable to those of normal XY male littermates. Our results show that maintenance of the ovarian phenotype is an active process throughout life. They might also have important medical implications for the understanding and treatment of some disorders of sexual development in children and premature menopause in women. For a video summary of this article, see the PaperFlick file with the Supplemental Data available online.

Original languageEnglish
Pages (from-to)1130-1142
Number of pages13
JournalCell
Volume139
Issue number6
DOIs
Publication statusPublished - Dec 11 2009
Externally publishedYes

Fingerprint

Ablation
Testis
Ovary
Genes
Mammals
Cell Lineage
Chromosomes
Testosterone
Transcription Factors
Premature Menopause
Theca Cells
Disorders of Sex Development
Ovarian Follicle
Leydig Cells
Granulosa Cells
Y Chromosome
Gonads
Up-Regulation
Maintenance
Phenotype

Keywords

  • DEVBIO
  • HUMDISEASE
  • SIGNALING

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Uhlenhaut, N. H., Jakob, S., Anlag, K., Eisenberger, T., Sekido, R., Kress, J., ... Treier, M. (2009). Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation. Cell, 139(6), 1130-1142. https://doi.org/10.1016/j.cell.2009.11.021

Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation. / Uhlenhaut, N. Henriette; Jakob, Susanne; Anlag, Katrin; Eisenberger, Tobias; Sekido, Ryohei; Kress, Jana; Treier, Anna Corina; Klugmann, Claudia; Klasen, Christian; Holter, Nadine I.; Riethmacher, Dieter; Schütz, Günther; Cooney, Austin J.; Lovell-Badge, Robin; Treier, Mathias.

In: Cell, Vol. 139, No. 6, 11.12.2009, p. 1130-1142.

Research output: Contribution to journalArticle

Uhlenhaut, NH, Jakob, S, Anlag, K, Eisenberger, T, Sekido, R, Kress, J, Treier, AC, Klugmann, C, Klasen, C, Holter, NI, Riethmacher, D, Schütz, G, Cooney, AJ, Lovell-Badge, R & Treier, M 2009, 'Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation', Cell, vol. 139, no. 6, pp. 1130-1142. https://doi.org/10.1016/j.cell.2009.11.021
Uhlenhaut NH, Jakob S, Anlag K, Eisenberger T, Sekido R, Kress J et al. Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation. Cell. 2009 Dec 11;139(6):1130-1142. https://doi.org/10.1016/j.cell.2009.11.021
Uhlenhaut, N. Henriette ; Jakob, Susanne ; Anlag, Katrin ; Eisenberger, Tobias ; Sekido, Ryohei ; Kress, Jana ; Treier, Anna Corina ; Klugmann, Claudia ; Klasen, Christian ; Holter, Nadine I. ; Riethmacher, Dieter ; Schütz, Günther ; Cooney, Austin J. ; Lovell-Badge, Robin ; Treier, Mathias. / Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation. In: Cell. 2009 ; Vol. 139, No. 6. pp. 1130-1142.
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