Abstract
The nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. Historically, averaged bulk analysis of viral particles has been the primary method to quantitatively investigate these parameters, though this neglects heterogeneity within populations. Here we analyse the properties of Junin virus particles using a sensitive flow virometry assay and further sort virions while conserving their infectiveness. This method allows us to characterize the relationship between infectivity, virus size and RNA content and to compare particles secreted by Vero cells with those from physiologically relevant human primary macrophages. Our study highlights significant differences in particle infectivity according to its nature, the type of producer cells and the lipid membrane composition at the budding site. Together, our results present the flow virometry assay as a powerful and versatile tool to define virus particle profiles.
| Original language | English |
|---|---|
| Article number | 6022 |
| Journal | Nature Communications |
| Volume | 6 |
| DOIs | |
| Publication status | Published - 2015 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Chemistry(all)
- Physics and Astronomy(all)
- Medicine(all)
Cite this
Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles. / Gaudin, Raphaël; Barteneva, Natasha S.
In: Nature Communications, Vol. 6, 6022, 2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles
AU - Gaudin, Raphaël
AU - Barteneva, Natasha S.
PY - 2015
Y1 - 2015
N2 - The nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. Historically, averaged bulk analysis of viral particles has been the primary method to quantitatively investigate these parameters, though this neglects heterogeneity within populations. Here we analyse the properties of Junin virus particles using a sensitive flow virometry assay and further sort virions while conserving their infectiveness. This method allows us to characterize the relationship between infectivity, virus size and RNA content and to compare particles secreted by Vero cells with those from physiologically relevant human primary macrophages. Our study highlights significant differences in particle infectivity according to its nature, the type of producer cells and the lipid membrane composition at the budding site. Together, our results present the flow virometry assay as a powerful and versatile tool to define virus particle profiles.
AB - The nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. Historically, averaged bulk analysis of viral particles has been the primary method to quantitatively investigate these parameters, though this neglects heterogeneity within populations. Here we analyse the properties of Junin virus particles using a sensitive flow virometry assay and further sort virions while conserving their infectiveness. This method allows us to characterize the relationship between infectivity, virus size and RNA content and to compare particles secreted by Vero cells with those from physiologically relevant human primary macrophages. Our study highlights significant differences in particle infectivity according to its nature, the type of producer cells and the lipid membrane composition at the budding site. Together, our results present the flow virometry assay as a powerful and versatile tool to define virus particle profiles.
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UR - http://www.scopus.com/inward/citedby.url?scp=84923098091&partnerID=8YFLogxK
U2 - 10.1038/ncomms7022
DO - 10.1038/ncomms7022
M3 - Article
C2 - 25641385
AN - SCOPUS:84923098091
VL - 6
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 6022
ER -