Source of oncofetal ED-B-containing fibronectin

Implications of production of both tumor and endothelial cells

Marta Midulla, Rakesh Verma, Massimo Pignatelli, Mary A. Ritter, Nigel S. Courtenay-Luck, Andrew J T George

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

ED-B fibronectin (FN) is a FN isoform derived from alternative splicing of the primary transcript of a single gene. Its expression on tumor stroma and neoformed tumor vasculature and its absence, with few exceptions, in normal adult tissues imply a prognostic and diagnostic value for ED-B FN. We investigated the location and source of ED-B FN because this will be of importance both in understanding its role in tumor development and in designing strategies to target this molecule. We have confirmed that ED-B FN is expressed in the majority of breast and colorectal carcinoma tissue samples, with strong immunohistochemical staining around the tumor cells and in the tumor stroma. No staining of tumor neovasculature was seen. ED-B FN is produced by a range of tumor and endothelial (both primary and transformed) cell lines, as detected by reverse transcription-PCR, but is not expressed at the plasma membrane. Strong expression of human ED-B FN is seen in tumor xenografts. These data indicate that neoplastic cells can act as the source of ED-B FN in tumors. The lack of cell surface expression on tumor cell lines has clear implications for the design of therapeutic strategies which target this molecule.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalCancer Research
Volume60
Issue number1
Publication statusPublished - Jan 1 2000
Externally publishedYes

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Fibronectins
Endothelial Cells
Neoplasms
Staining and Labeling
Transformed Cell Line
Alternative Splicing
Tumor Cell Line
Heterografts
Reverse Transcription
Colorectal Neoplasms
Protein Isoforms
Cell Membrane
Breast Neoplasms
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Midulla, M., Verma, R., Pignatelli, M., Ritter, M. A., Courtenay-Luck, N. S., & George, A. J. T. (2000). Source of oncofetal ED-B-containing fibronectin: Implications of production of both tumor and endothelial cells. Cancer Research, 60(1), 164-169.

Source of oncofetal ED-B-containing fibronectin : Implications of production of both tumor and endothelial cells. / Midulla, Marta; Verma, Rakesh; Pignatelli, Massimo; Ritter, Mary A.; Courtenay-Luck, Nigel S.; George, Andrew J T.

In: Cancer Research, Vol. 60, No. 1, 01.01.2000, p. 164-169.

Research output: Contribution to journalArticle

Midulla, M, Verma, R, Pignatelli, M, Ritter, MA, Courtenay-Luck, NS & George, AJT 2000, 'Source of oncofetal ED-B-containing fibronectin: Implications of production of both tumor and endothelial cells', Cancer Research, vol. 60, no. 1, pp. 164-169.
Midulla, Marta ; Verma, Rakesh ; Pignatelli, Massimo ; Ritter, Mary A. ; Courtenay-Luck, Nigel S. ; George, Andrew J T. / Source of oncofetal ED-B-containing fibronectin : Implications of production of both tumor and endothelial cells. In: Cancer Research. 2000 ; Vol. 60, No. 1. pp. 164-169.
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