Abstract
In 1983 we discovered a phenomenon of the total fragmentation (fission) of the entire mitochondrial population in a cell following the exposure to a mitochondrial inhibitor. Further experiments showed that a wide range of substances acting on mitochondria can cause the transformation of filamentous structures to small globular vesicles (the thread–grain transition). Fragmentation of mitochondria clearly begins with the formation of a septum formed by the inner mitochondrial membranes, leading to the division of mitochondria into a number of compartments, not necessarily of the same configuration. Visible signs of fragmentation of the mitochondrial reticulum appeared after an hour of the exposure to mitochondrial inhibitors; the process of mitochondrial fission was markedly accelerated and developed within seconds when the cells stained with fluorescent probes were exposed to excitation light and were affected photodynamically. The crucial role of reactive oxygen species in the initiation of the process of mitochondrial fission is proposed. Before the formation of septa, local enlightenment of mitochondrial matrix (local swelling) can be observed, which indicates the possibility of the existence of intramitochondrial skeleton that maintains the shape of the mitochondria. The septum formation and fragmentation gives rise to different populations of mitochondria. Apparently, this is a part of the mechanism of mitochondrial quality control and elimination of damaged mitochondria.
| Original language | English |
|---|---|
| Pages (from-to) | 278-284 |
| Number of pages | 7 |
| Journal | Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology |
| Volume | 9 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 1 2015 |
| Externally published | Yes |
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Keywords
- fission
- fragmentation
- mitochondria
- reactive oxygen species
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
Cite this
Specific issues of mitochondrial fragmentation (Fission). / Zorov, D. B.; Vorobjev, I. A.; Plotnikov, E. Y.; Silachev, D. N.; Zorova, L. D.; Pevzner, I. B.; Babenko, V. A.; Zorov, S. D.; Jankauskas, S. S.; Popkov, V. A.
In: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, Vol. 9, No. 4, 01.10.2015, p. 278-284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Specific issues of mitochondrial fragmentation (Fission)
AU - Zorov, D. B.
AU - Vorobjev, I. A.
AU - Plotnikov, E. Y.
AU - Silachev, D. N.
AU - Zorova, L. D.
AU - Pevzner, I. B.
AU - Babenko, V. A.
AU - Zorov, S. D.
AU - Jankauskas, S. S.
AU - Popkov, V. A.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - In 1983 we discovered a phenomenon of the total fragmentation (fission) of the entire mitochondrial population in a cell following the exposure to a mitochondrial inhibitor. Further experiments showed that a wide range of substances acting on mitochondria can cause the transformation of filamentous structures to small globular vesicles (the thread–grain transition). Fragmentation of mitochondria clearly begins with the formation of a septum formed by the inner mitochondrial membranes, leading to the division of mitochondria into a number of compartments, not necessarily of the same configuration. Visible signs of fragmentation of the mitochondrial reticulum appeared after an hour of the exposure to mitochondrial inhibitors; the process of mitochondrial fission was markedly accelerated and developed within seconds when the cells stained with fluorescent probes were exposed to excitation light and were affected photodynamically. The crucial role of reactive oxygen species in the initiation of the process of mitochondrial fission is proposed. Before the formation of septa, local enlightenment of mitochondrial matrix (local swelling) can be observed, which indicates the possibility of the existence of intramitochondrial skeleton that maintains the shape of the mitochondria. The septum formation and fragmentation gives rise to different populations of mitochondria. Apparently, this is a part of the mechanism of mitochondrial quality control and elimination of damaged mitochondria.
AB - In 1983 we discovered a phenomenon of the total fragmentation (fission) of the entire mitochondrial population in a cell following the exposure to a mitochondrial inhibitor. Further experiments showed that a wide range of substances acting on mitochondria can cause the transformation of filamentous structures to small globular vesicles (the thread–grain transition). Fragmentation of mitochondria clearly begins with the formation of a septum formed by the inner mitochondrial membranes, leading to the division of mitochondria into a number of compartments, not necessarily of the same configuration. Visible signs of fragmentation of the mitochondrial reticulum appeared after an hour of the exposure to mitochondrial inhibitors; the process of mitochondrial fission was markedly accelerated and developed within seconds when the cells stained with fluorescent probes were exposed to excitation light and were affected photodynamically. The crucial role of reactive oxygen species in the initiation of the process of mitochondrial fission is proposed. Before the formation of septa, local enlightenment of mitochondrial matrix (local swelling) can be observed, which indicates the possibility of the existence of intramitochondrial skeleton that maintains the shape of the mitochondria. The septum formation and fragmentation gives rise to different populations of mitochondria. Apparently, this is a part of the mechanism of mitochondrial quality control and elimination of damaged mitochondria.
KW - fission
KW - fragmentation
KW - mitochondria
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84951039773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84951039773&partnerID=8YFLogxK
U2 - 10.1134/S1990747815050219
DO - 10.1134/S1990747815050219
M3 - Article
AN - SCOPUS:84951039773
VL - 9
SP - 278
EP - 284
JO - Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
JF - Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
SN - 1990-7478
IS - 4
ER -