Spectral and imaging flow cytometry in phytoplankton research

Veronika Dashkova, Jeff Clapper, Ivan Vorobyev, Natalie Barteneva

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Spectral and imaging flow cytometry are emerging technologies that allow quantifying spectral, fluorescent, and/or morphological parameters of heterogeneous cellular populations. The protocol describes a detailed step-by-step analysis of microalgae using these techniques and examples from our laboratory (Aphanizomenon sp., Cryptomonas pyrenoidifera, and Chlorella sp.). Moreover, the chapter will be helpful to scientists who want to perform spectral flow cytometry and apply principal component analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-95
Number of pages13
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1745
ISSN (Print)1064-3745

Fingerprint

Phytoplankton
Aphanizomenon
Flow Cytometry
Cryptophyta
Microalgae
Chlorella
Principal Component Analysis
Research
Technology
Population

Keywords

  • Imaging flow cytometry
  • Microalgae
  • Population heterogeneity
  • Programmed cell death
  • Spectral flow cytometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Dashkova, V., Clapper, J., Vorobyev, I., & Barteneva, N. (2018). Spectral and imaging flow cytometry in phytoplankton research. In Methods in Molecular Biology (pp. 83-95). (Methods in Molecular Biology; Vol. 1745). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7680-5_5

Spectral and imaging flow cytometry in phytoplankton research. / Dashkova, Veronika; Clapper, Jeff; Vorobyev, Ivan; Barteneva, Natalie.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 83-95 (Methods in Molecular Biology; Vol. 1745).

Research output: Chapter in Book/Report/Conference proceedingChapter

Dashkova, V, Clapper, J, Vorobyev, I & Barteneva, N 2018, Spectral and imaging flow cytometry in phytoplankton research. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1745, Humana Press Inc., pp. 83-95. https://doi.org/10.1007/978-1-4939-7680-5_5
Dashkova V, Clapper J, Vorobyev I, Barteneva N. Spectral and imaging flow cytometry in phytoplankton research. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 83-95. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7680-5_5
Dashkova, Veronika ; Clapper, Jeff ; Vorobyev, Ivan ; Barteneva, Natalie. / Spectral and imaging flow cytometry in phytoplankton research. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 83-95 (Methods in Molecular Biology).
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