Spectral and imaging flow cytometry in phytoplankton research

Veronika Dashkova, Jeff Clapper, Ivan Vorobyev, Natalie Barteneva

Research output: Chapter in Book/Report/Conference proceedingChapter


Spectral and imaging flow cytometry are emerging technologies that allow quantifying spectral, fluorescent, and/or morphological parameters of heterogeneous cellular populations. The protocol describes a detailed step-by-step analysis of microalgae using these techniques and examples from our laboratory (Aphanizomenon sp., Cryptomonas pyrenoidifera, and Chlorella sp.). Moreover, the chapter will be helpful to scientists who want to perform spectral flow cytometry and apply principal component analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Imaging flow cytometry
  • Microalgae
  • Population heterogeneity
  • Programmed cell death
  • Spectral flow cytometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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