Abstract
Nitric oxide (NO) has been shown to be an important mediator in vasodilation, neurotransmission and cellular cytotoxicity. We investigated a new series of nitronylnitroxyl radicals (NNR) as spin traps for NO. It was found these radicals react with NO with rate constants of about 104 M-1c-1 forming stable iminonitroxides with dramatic changes in EPR spectra. To overcome fast reduction of the radicals (a few seconds in rat cerebella cytosol), NNR with charged trimethylammoniophenyl group (Ib) was incorporated into the inner volume of large unilamellar phosphatidylcholine liposomes. In this case the reduction of the radical Ib in rat cerebella cytosol is slow (ca. 1% per min). The rate of NO production by NO synthase from rat cerebellum measured by NNR, Ib, is in a reasonable agreement with that obtained by spectrophotometric method.
| Original language | English |
|---|---|
| Pages (from-to) | 195-203 |
| Number of pages | 9 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 202 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 15 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Spin Trapping of Nitric Oxide by Nitronylnitroxides : Measurement of the Activity of NO Synthase from Rat Cerebellum. / Woldman, Y. Y.; Khramtsov, V. V.; Grigorev, I. A.; Kiriljuk, I. A.; Utepbergenov, D. I.
In: Biochemical and Biophysical Research Communications, Vol. 202, No. 1, 15.07.1994, p. 195-203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spin Trapping of Nitric Oxide by Nitronylnitroxides
T2 - Biochemical and Biophysical Research Communications
AU - Woldman, Y. Y.
AU - Khramtsov, V. V.
AU - Grigorev, I. A.
AU - Kiriljuk, I. A.
AU - Utepbergenov, D. I.
PY - 1994/7/15
Y1 - 1994/7/15
N2 - Nitric oxide (NO) has been shown to be an important mediator in vasodilation, neurotransmission and cellular cytotoxicity. We investigated a new series of nitronylnitroxyl radicals (NNR) as spin traps for NO. It was found these radicals react with NO with rate constants of about 104 M-1c-1 forming stable iminonitroxides with dramatic changes in EPR spectra. To overcome fast reduction of the radicals (a few seconds in rat cerebella cytosol), NNR with charged trimethylammoniophenyl group (Ib) was incorporated into the inner volume of large unilamellar phosphatidylcholine liposomes. In this case the reduction of the radical Ib in rat cerebella cytosol is slow (ca. 1% per min). The rate of NO production by NO synthase from rat cerebellum measured by NNR, Ib, is in a reasonable agreement with that obtained by spectrophotometric method.
AB - Nitric oxide (NO) has been shown to be an important mediator in vasodilation, neurotransmission and cellular cytotoxicity. We investigated a new series of nitronylnitroxyl radicals (NNR) as spin traps for NO. It was found these radicals react with NO with rate constants of about 104 M-1c-1 forming stable iminonitroxides with dramatic changes in EPR spectra. To overcome fast reduction of the radicals (a few seconds in rat cerebella cytosol), NNR with charged trimethylammoniophenyl group (Ib) was incorporated into the inner volume of large unilamellar phosphatidylcholine liposomes. In this case the reduction of the radical Ib in rat cerebella cytosol is slow (ca. 1% per min). The rate of NO production by NO synthase from rat cerebellum measured by NNR, Ib, is in a reasonable agreement with that obtained by spectrophotometric method.
UR - http://www.scopus.com/inward/record.url?scp=0028022842&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028022842&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.1912
DO - 10.1006/bbrc.1994.1912
M3 - Article
VL - 202
SP - 195
EP - 203
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -