Standard strain of human ocular keratocytes

Simona Dropcova, Steven P. Denyer, Andrew W. Lloyd, Paul R. Gard, Geoff W. Hanlon, Sergei V. Mikhalovsky, Susan Sandeman, Cedric J. Olliff, Richard G.A. Faragher

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The ability of an injured cornea to regenerate from deep tissue trauma is largely due to wound healing processes mediated by the surviving stromal keratocytes. Despite the importance of the wound healing process, and the ease with which keratocytes can be grown in tissue culture, a standardised strain of the cells has never been made available. Accordingly, this study reports a strain of human embryonic keratocytes, designated EK1.BR as a research tool for the ophthalmic community. EK1.BR has been characterised with respect to life-span, fraction of diving cells and maintenance of a keratocyte phenotype in culture. It is hoped that these cells will prove useful in the in vitro study of stromal wound healing and the characterisation of keratocyte gene expression.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalOphthalmic Research
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Ageing
  • Cornea
  • Keratocyte
  • Proliferation
  • Senescence
  • Wound healing

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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