Stimulation of neuronal neurite outgrowth using functionalized carbon nanotubes

K. Matsumoto, C. Sato, Y. Naka, R. Whitby, N. Shimizu

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Low concentrations (0.11-1.7μgml- 1) of functionalized carbon nanotubes (CNTs), which are multi-walled CNTs modified by amino groups, when added with nerve growth factor (NGF), promoted outgrowth of neuronal neurites in dorsal root ganglion (DRG) neurons and rat pheochromocytoma cell line PC12h cells in culture media. The quantity of active extracellular signal-regulated kinase (ERK) was higher after the addition of both 0.85 μgml- 1 CNTs and NGF than that with NGF alone. CNTs increased the number of cells with neurite outgrowth in DRG neurons and PC12h cells after the inhibition of the ERK signaling pathway using a mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) inhibitor. Active ERK proteins were detected in MEK inhibitor-treated neurons after the addition of CNTs to the culture medium. These results demonstrate that CNTs may stimulate neurite outgrowth by activation of the ERK signaling pathway. Thus, CNTs are biocompatible and are promising candidates for biological applications and devices.

Original languageEnglish
Article number115101
JournalNanotechnology
Volume21
Issue number11
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Carbon Nanotubes
Carbon nanotubes
Extracellular Signal-Regulated MAP Kinases
Nerve Growth Factor
Neurons
Culture Media
Proteins
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Cell culture
Rats
Phosphotransferases
Chemical activation
Cells
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Stimulation of neuronal neurite outgrowth using functionalized carbon nanotubes. / Matsumoto, K.; Sato, C.; Naka, Y.; Whitby, R.; Shimizu, N.

In: Nanotechnology, Vol. 21, No. 11, 115101, 2010.

Research output: Contribution to journalArticle

Matsumoto, K. ; Sato, C. ; Naka, Y. ; Whitby, R. ; Shimizu, N. / Stimulation of neuronal neurite outgrowth using functionalized carbon nanotubes. In: Nanotechnology. 2010 ; Vol. 21, No. 11.
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