Structure of gene mts1, transcribed in metastatic mouse tumor cells

E. M. Tulchinsky, M. S. Grigorian, A. K. Ebralidze, N. I. Milshina, E. M. Lukanidin

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Different oncogenes are implicated in the genesis of tumors. However, little is known so far about the genes which are activated at the latest stages of tumor progression. While studying two genetically related mouse lines, highly metastatic CSML-100 and nearly nonmetastatic CSML-0, we have cloned the cDNA of the gene, mts1, which is specifically expressed in different metastatic cells. The gene contains an open reading frame of 101 amino acids and shows homology with a family of Ca2+-binding proteins. Here, we present data on the structure of a 17-kb genomic clone of mts1 with surrounding sequences. The gene contains two introns and three exons. The mts1 upstream region has been cloned in a plasmid containing the cat gene. The results of transient expression of the mtsl-cat plasmid in NIH3T3 cells indicate the presence of a transcription regulator of mts1.

Original languageEnglish
Pages (from-to)219-223
Number of pages5
JournalGene
Volume87
Issue number2
DOIs
Publication statusPublished - Mar 15 1990
Externally publishedYes

Keywords

  • calcium-binding protein
  • CAT assay
  • exon
  • intron
  • phage λ vectors
  • promoter
  • Recombinant DNA
  • tissue-specific expression

ASJC Scopus subject areas

  • Genetics

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