Structure of the chromatin binding (chromo) domain from mouse modifier protein 1

L J Ball, N V Murzina, R W Broadhurst, A R Raine, S J Archer, F J Stott, A G Murzin, P B Singh, P J Domaille, E D Laue

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The structure of a chromatin binding domain from mouse chromatin modifier protein 1 (MoMOD1) was determined using nuclear magnetic resonance (NMR) spectroscopy. The protein consists of an N-terminal three-stranded anti-parallel beta-sheet which folds against a C-terminal alpha-helix. The structure reveals an unexpected homology to two archaebacterial DNA binding proteins which are also involved in chromatin structure. Structural comparisons suggest that chromo domains, of which more than 40 are now known, act as protein interaction motifs and that the MoMOD1 protein acts as an adaptor mediating interactions between different proteins.

Original languageEnglish
Pages (from-to)2473-81
Number of pages9
JournalEMBO Journal
Volume16
Issue number9
DOIs
Publication statusPublished - May 1 1997

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Chromatin
Proteins
Protein Interaction Domains and Motifs
DNA-Binding Proteins
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy

Keywords

  • Amino Acid Sequence
  • Animals
  • Archaeal Proteins
  • Bacterial Proteins
  • Binding Sites
  • Carrier Proteins
  • Chromatin
  • Chromatography, High Pressure Liquid
  • Chromosomal Proteins, Non-Histone
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Magnetic Resonance Spectroscopy
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Solutions
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Ball, L. J., Murzina, N. V., Broadhurst, R. W., Raine, A. R., Archer, S. J., Stott, F. J., ... Laue, E. D. (1997). Structure of the chromatin binding (chromo) domain from mouse modifier protein 1. EMBO Journal, 16(9), 2473-81. https://doi.org/10.1093/emboj/16.9.2473

Structure of the chromatin binding (chromo) domain from mouse modifier protein 1. / Ball, L J; Murzina, N V; Broadhurst, R W; Raine, A R; Archer, S J; Stott, F J; Murzin, A G; Singh, P B; Domaille, P J; Laue, E D.

In: EMBO Journal, Vol. 16, No. 9, 01.05.1997, p. 2473-81.

Research output: Contribution to journalArticle

Ball, LJ, Murzina, NV, Broadhurst, RW, Raine, AR, Archer, SJ, Stott, FJ, Murzin, AG, Singh, PB, Domaille, PJ & Laue, ED 1997, 'Structure of the chromatin binding (chromo) domain from mouse modifier protein 1', EMBO Journal, vol. 16, no. 9, pp. 2473-81. https://doi.org/10.1093/emboj/16.9.2473
Ball LJ, Murzina NV, Broadhurst RW, Raine AR, Archer SJ, Stott FJ et al. Structure of the chromatin binding (chromo) domain from mouse modifier protein 1. EMBO Journal. 1997 May 1;16(9):2473-81. https://doi.org/10.1093/emboj/16.9.2473
Ball, L J ; Murzina, N V ; Broadhurst, R W ; Raine, A R ; Archer, S J ; Stott, F J ; Murzin, A G ; Singh, P B ; Domaille, P J ; Laue, E D. / Structure of the chromatin binding (chromo) domain from mouse modifier protein 1. In: EMBO Journal. 1997 ; Vol. 16, No. 9. pp. 2473-81.
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