Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism

Douglas A. Hattendorf, Anna Andreeva, Akanksha Gangar, Patrick J. Brennwald, William I. Weis

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Polarized exocytosis requires coordination between the actin cytoskeleton and the exocytic machinery responsible for fusion of secretory vesicles at specific sites on the plasma membrane. Fusion requires formation of a complex between a vesicle-bound R-SNARE and plasma membrane Qa, Qb and Qc SNARE proteins. Proteins in the lethal giant larvae protein family, including lethal giant larvae and tomosyn in metazoans and Sro7 in yeast, interact with Q-SNAREs and are emerging as key regulators of polarized exocytosis. The crystal structure of Sro7 reveals two seven-bladed WD40 β-propellers followed by a 60-residue-long 'tail', which is bound to the surface of the amino-terminal propeller. Deletion of the Sro7 tail enables binding to the Qbc SNARE region of Sec9 and this interaction inhibits SNARE complex assembly. The N-terminal domain of Sec9 provides a second, high-affinity Sro7 interaction that is unaffected by the tail. The results suggest that Sro7 acts as an allosteric regulator of exocytosis through interactions with factors that control the tail. Sequence alignments indicate that lethal giant larvae and tomosyn have a two-β-propeller fold similar to that of Sro7, but only tomosyn appears to retain the regulatory tail.

Original languageEnglish
Pages (from-to)567-571
Number of pages5
JournalNature
Volume446
Issue number7135
DOIs
Publication statusPublished - Mar 29 2007
Externally publishedYes

Fingerprint

SNARE Proteins
Fungal Proteins
Exocytosis
Larva
Qc-SNARE Proteins
Qb-SNARE Proteins
Q-SNARE Proteins
Cell Membrane
Qa-SNARE Proteins
Sequence Alignment
Secretory Vesicles
Actin Cytoskeleton
Membrane Proteins
Proteins
Yeasts

ASJC Scopus subject areas

  • General

Cite this

Hattendorf, D. A., Andreeva, A., Gangar, A., Brennwald, P. J., & Weis, W. I. (2007). Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism. Nature, 446(7135), 567-571. https://doi.org/10.1038/nature05635

Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism. / Hattendorf, Douglas A.; Andreeva, Anna; Gangar, Akanksha; Brennwald, Patrick J.; Weis, William I.

In: Nature, Vol. 446, No. 7135, 29.03.2007, p. 567-571.

Research output: Contribution to journalArticle

Hattendorf, DA, Andreeva, A, Gangar, A, Brennwald, PJ & Weis, WI 2007, 'Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism', Nature, vol. 446, no. 7135, pp. 567-571. https://doi.org/10.1038/nature05635
Hattendorf, Douglas A. ; Andreeva, Anna ; Gangar, Akanksha ; Brennwald, Patrick J. ; Weis, William I. / Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism. In: Nature. 2007 ; Vol. 446, No. 7135. pp. 567-571.
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