Study of childhood renal tumours using a monoclonal antibody to Tamm-Horsfall protein

S. Kumar, H. B. Marsden, B. Jasani, P. Kumar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A monoclonal antibody to Tamm-Horsfall glycoprotein was used for the immunolocalisation of Tamm-Horsfall protein in formalin fixed, paraffin embedded tissue sections of childhood renal tumours, normal children's kidneys, and human fetal kidneys. The procedure was a dinitrophenyl hapten sandwich staining method. The antibody, diluted 1/100000, gave a very strong and specific staining of the loop of Henle and distal tubules of normal and fetal kidneys. No staining was seen in Wilms' tumour, mesoblastic nephroma, and bone metastasing renal tumour of childhood. In contrast, two of seven renal carcinomas and three of four rhabdoid renal tumours were positive for Tamm-Horsfall protein.

Original languageEnglish
Pages (from-to)1456-1462
Number of pages7
JournalJournal of Clinical Pathology
Volume40
Issue number12
DOIs
Publication statusPublished - 1987
Externally publishedYes

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Uromodulin
Monoclonal Antibodies
Kidney
Neoplasms
Staining and Labeling
Mesoblastic Nephroma
Rhabdoid Tumor
Loop of Henle
Wilms Tumor
Haptens
Paraffin
Formaldehyde
Carcinoma
Bone and Bones
Antibodies

ASJC Scopus subject areas

  • Health Policy
  • Pathology and Forensic Medicine

Cite this

Study of childhood renal tumours using a monoclonal antibody to Tamm-Horsfall protein. / Kumar, S.; Marsden, H. B.; Jasani, B.; Kumar, P.

In: Journal of Clinical Pathology, Vol. 40, No. 12, 1987, p. 1456-1462.

Research output: Contribution to journalArticle

Kumar, S. ; Marsden, H. B. ; Jasani, B. ; Kumar, P. / Study of childhood renal tumours using a monoclonal antibody to Tamm-Horsfall protein. In: Journal of Clinical Pathology. 1987 ; Vol. 40, No. 12. pp. 1456-1462.
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