T lymphocyte subsets implicated in cytotoxicity in autologous hepatocytes in chronic active hepatitis patients with active viral replication

G. F. Stefanini; Maura Mazzetti; P. Zunarelli; M. Baraldini; M. Pignatelli; G. W. Canonica; F. Miglio; G. Gasbarrini

doi:10.1016/0090-1229(86)90024-3

T lymphocyte subsets implicated in cytotoxicity in autologous hepatocytes in chronic active hepatitis patients with active viral replication

G. F. Stefanini, Maura Mazzetti, P. Zunarelli, M. Baraldini, M. Pignatelli, G. W. Canonica, F. Miglio, G. Gasbarrini

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We investigated inhibitory effect of various monoclonal antibodies on T-cell-mediated cytotoxicity against autologous hepatocytes in 24 patients with hepatitis B surface antigen/hepatitis B e antigen (HB_sAg HB_eAg)-positive chronic active hepatitis. A significant reduction of cytotoxicity index occurred after preincubation of T lymphocytes with anti-Leu 7 (killer-natural killer cells), D1 12 (la-positive cells), 5 9 (restricted helper/inducer cells), and MLR4 ("activated" and radiosensitive helper cells) monoclonal antibodies (MAb). Anti-Leu 2a (cytotoxic/suppressor cells) and anti-Leu 3a (helper/inducer cells) MAb did not affect cytotoxic activity. This finding supports the hypothesis that the T cytotoxic reaction in this in vitro system is probably due to two mechanisms: first, spontaneous cell membrane cytotoxicity sustained by anti-Leu-7-positive lymphocytes; and second, specific cytotoxicity mediated by activated Ia-positive cells. We also found that the presence of helper/inducer cells ( 5 9 positive) appears to be a prerequisite for the cytotoxic reaction.

Original language	English
Pages (from-to)	214-223
Number of pages	10
Journal	Clinical Immunology and Immunopathology
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/0090-1229(86)90024-3
Publication status	Published - Aug 1986

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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Stefanini, G. F., Mazzetti, M., Zunarelli, P., Baraldini, M., Pignatelli, M., Canonica, G. W., Miglio, F., & Gasbarrini, G. (1986). T lymphocyte subsets implicated in cytotoxicity in autologous hepatocytes in chronic active hepatitis patients with active viral replication. Clinical Immunology and Immunopathology, 40(2), 214-223. https://doi.org/10.1016/0090-1229(86)90024-3

T lymphocyte subsets implicated in cytotoxicity in autologous hepatocytes in chronic active hepatitis patients with active viral replication. / Stefanini, G. F.; Mazzetti, Maura; Zunarelli, P.; Baraldini, M.; Pignatelli, M.; Canonica, G. W.; Miglio, F.; Gasbarrini, G.

In: Clinical Immunology and Immunopathology, Vol. 40, No. 2, 08.1986, p. 214-223.

Research output: Contribution to journal › Article › peer-review

Stefanini, GF, Mazzetti, M, Zunarelli, P, Baraldini, M, Pignatelli, M, Canonica, GW, Miglio, F & Gasbarrini, G 1986, 'T lymphocyte subsets implicated in cytotoxicity in autologous hepatocytes in chronic active hepatitis patients with active viral replication', Clinical Immunology and Immunopathology, vol. 40, no. 2, pp. 214-223. https://doi.org/10.1016/0090-1229(86)90024-3

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abstract = "We investigated inhibitory effect of various monoclonal antibodies on T-cell-mediated cytotoxicity against autologous hepatocytes in 24 patients with hepatitis B surface antigen/hepatitis B e antigen (HBsAg HBeAg)-positive chronic active hepatitis. A significant reduction of cytotoxicity index occurred after preincubation of T lymphocytes with anti-Leu 7 (killer-natural killer cells), D1 12 (la-positive cells), 5 9 (restricted helper/inducer cells), and MLR4 ({"}activated{"} and radiosensitive helper cells) monoclonal antibodies (MAb). Anti-Leu 2a (cytotoxic/suppressor cells) and anti-Leu 3a (helper/inducer cells) MAb did not affect cytotoxic activity. This finding supports the hypothesis that the T cytotoxic reaction in this in vitro system is probably due to two mechanisms: first, spontaneous cell membrane cytotoxicity sustained by anti-Leu-7-positive lymphocytes; and second, specific cytotoxicity mediated by activated Ia-positive cells. We also found that the presence of helper/inducer cells ( 5 9 positive) appears to be a prerequisite for the cytotoxic reaction.",

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AB - We investigated inhibitory effect of various monoclonal antibodies on T-cell-mediated cytotoxicity against autologous hepatocytes in 24 patients with hepatitis B surface antigen/hepatitis B e antigen (HBsAg HBeAg)-positive chronic active hepatitis. A significant reduction of cytotoxicity index occurred after preincubation of T lymphocytes with anti-Leu 7 (killer-natural killer cells), D1 12 (la-positive cells), 5 9 (restricted helper/inducer cells), and MLR4 ("activated" and radiosensitive helper cells) monoclonal antibodies (MAb). Anti-Leu 2a (cytotoxic/suppressor cells) and anti-Leu 3a (helper/inducer cells) MAb did not affect cytotoxic activity. This finding supports the hypothesis that the T cytotoxic reaction in this in vitro system is probably due to two mechanisms: first, spontaneous cell membrane cytotoxicity sustained by anti-Leu-7-positive lymphocytes; and second, specific cytotoxicity mediated by activated Ia-positive cells. We also found that the presence of helper/inducer cells ( 5 9 positive) appears to be a prerequisite for the cytotoxic reaction.

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