Abstract
We investigated inhibitory effect of various monoclonal antibodies on T-cell-mediated cytotoxicity against autologous hepatocytes in 24 patients with hepatitis B surface antigen/hepatitis B e antigen (HBsAg HBeAg)-positive chronic active hepatitis. A significant reduction of cytotoxicity index occurred after preincubation of T lymphocytes with anti-Leu 7 (killer-natural killer cells), D1 12 (la-positive cells), 5 9 (restricted helper/inducer cells), and MLR4 ("activated" and radiosensitive helper cells) monoclonal antibodies (MAb). Anti-Leu 2a (cytotoxic/suppressor cells) and anti-Leu 3a (helper/inducer cells) MAb did not affect cytotoxic activity. This finding supports the hypothesis that the T cytotoxic reaction in this in vitro system is probably due to two mechanisms: first, spontaneous cell membrane cytotoxicity sustained by anti-Leu-7-positive lymphocytes; and second, specific cytotoxicity mediated by activated Ia-positive cells. We also found that the presence of helper/inducer cells ( 5 9 positive) appears to be a prerequisite for the cytotoxic reaction.
Original language | English |
---|---|
Pages (from-to) | 214-223 |
Number of pages | 10 |
Journal | Clinical Immunology and Immunopathology |
Volume | 40 |
Issue number | 2 |
DOIs | |
Publication status | Published - Aug 1986 |
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine
- Immunology