Abstract
The yeast TOR2 gene encodes an essential 282 kd phosphatidylinositol (PI) 3-kinase homolog. TOR2 is related to the catalytic subunit of bovine PI 3-kinase and to yeast VPS34, a vacuolar sorting protein also shown to have PI 3-kinase activity. The immunosuppressant rapamycin most likely acts by inhibiting PI kinase activity because TOR2 mutations confer resistance to rapamycin and because a TOR1 TOR2 double disruption (TOR1 is a nonessential TOR2 homolog) confers G1 arrest, as does rapamycin. Our results further suggest that 3-phosphorylated phosphoinositides, whose physiological significance has not been determined, are an important signal in cell cycle activation. In yeast, this signal may act in a signal transduction pathway similar to the interleukin-2 signal transduction pathway in T cells.
| Original language | English |
|---|---|
| Pages (from-to) | 585-596 |
| Number of pages | 12 |
| Journal | Cell |
| Volume | 73 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 7 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression. / Kunz, Jeannette; Henriquez, Ruben; Schneider, Ulrich; Deuter-Reinhard, Maja; Movva, N. Rao; Hall, Michael N.
In: Cell, Vol. 73, No. 3, 07.05.1993, p. 585-596.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression
AU - Kunz, Jeannette
AU - Henriquez, Ruben
AU - Schneider, Ulrich
AU - Deuter-Reinhard, Maja
AU - Movva, N. Rao
AU - Hall, Michael N.
PY - 1993/5/7
Y1 - 1993/5/7
N2 - The yeast TOR2 gene encodes an essential 282 kd phosphatidylinositol (PI) 3-kinase homolog. TOR2 is related to the catalytic subunit of bovine PI 3-kinase and to yeast VPS34, a vacuolar sorting protein also shown to have PI 3-kinase activity. The immunosuppressant rapamycin most likely acts by inhibiting PI kinase activity because TOR2 mutations confer resistance to rapamycin and because a TOR1 TOR2 double disruption (TOR1 is a nonessential TOR2 homolog) confers G1 arrest, as does rapamycin. Our results further suggest that 3-phosphorylated phosphoinositides, whose physiological significance has not been determined, are an important signal in cell cycle activation. In yeast, this signal may act in a signal transduction pathway similar to the interleukin-2 signal transduction pathway in T cells.
AB - The yeast TOR2 gene encodes an essential 282 kd phosphatidylinositol (PI) 3-kinase homolog. TOR2 is related to the catalytic subunit of bovine PI 3-kinase and to yeast VPS34, a vacuolar sorting protein also shown to have PI 3-kinase activity. The immunosuppressant rapamycin most likely acts by inhibiting PI kinase activity because TOR2 mutations confer resistance to rapamycin and because a TOR1 TOR2 double disruption (TOR1 is a nonessential TOR2 homolog) confers G1 arrest, as does rapamycin. Our results further suggest that 3-phosphorylated phosphoinositides, whose physiological significance has not been determined, are an important signal in cell cycle activation. In yeast, this signal may act in a signal transduction pathway similar to the interleukin-2 signal transduction pathway in T cells.
UR - http://www.scopus.com/inward/record.url?scp=0027311858&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027311858&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(93)90144-F
DO - 10.1016/0092-8674(93)90144-F
M3 - Article
C2 - 8387896
AN - SCOPUS:0027311858
VL - 73
SP - 585
EP - 596
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -