Targeted binding of PEG-lipid modified polymer ultrasound contrast agents with tiered surface architecture

Wynter J. Duncanson, Kelleny Oum, John R. Eisenbrey, Robin O. Cleveland, Margaret A. Wheatley, Joyce Y. Wong

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

In order for site-directed polymer ultrasound contrast agents (UCAs) to provide acoustic enhancement at disease sites to distinguish normal tissue from diseased tissue, the surface of these agents must be functionalized with mixtures of grafted polymers. Here a combination of longer liganded polyethylene glycol (PEG)-lipids and shorter unliganded PEG-lipids were introduced into the oil phase of a modified solvent evaporation double emulsion method for preparing UCAs. UCAs with different lengths of both liganded and unliganded lipids were imaged under 7.5MHz ultrasound. The B-mode image brightness of the mixed PEG-lipid UCAs was within 1 dB the brightness of the unliganded surface. After 15 min of continuous insonation, 70% of the contrast signal remained. The peptide arginine-glycine-aspartic acid (RGD) was added to the surface of these UCAs through a biotin-avidin linkage and binding was assessed under static and shear conditions. Binding was significant after 30 min of static incubation and the adherence of the UCA increased under shear flow from 3 UCA/cell (static) to 5 UCA/cell (shear).

Original languageEnglish
Pages (from-to)501-506
Number of pages6
JournalBiotechnology and Bioengineering
Volume106
Issue number3
DOIs
Publication statusPublished - Jun 15 2010

Keywords

  • Binding
  • In vitro
  • PEG-lipid
  • Polymer
  • Ultrasound contrast agent

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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