The amplification of polymerized diaminobenzidine with physical developers: Sensitizing effects of transition metal salts and sulphide

C. J. Von Ruhland, B. Jasani

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Amplification of metal-complexed polymerized diaminobenzidine by two light-insensitive physical developers was systematically examined in a dot blot model system following either polymerizing diaminobenzidine in the presence of transition metal salts or applying the metal salts post-diaminobenzidine polymerization. The effect of sodium sulphide treatment on subsequent amplification was also investigated. Those metal-diaminobenzidine complexes that facilitated the most powerful amplification were subsequently tested in an immunohistochemical setting. The most dramatic amplification of polymerized diaminobenzidine was observed following its post-polymerization treatment with salts of platinum alone, or gold or vanadium with subsequent sulphide treatment, and allowed previously invisible quantities of polymerized diaminobenzidine to be clearly seen. Three other transition metal salts also improved the amplification of polymerized diaminobenzidine but to a lesser degree, namely nickel alone, and silver or rhodium with subsequent sulphide treatment. Sensitivity was comparable with the colloidal gold-silver amplification system.

Original languageEnglish
Pages (from-to)111-122
Number of pages12
JournalJournal of Microscopy
Volume238
Issue number2
DOIs
Publication statusPublished - May 1 2010

Keywords

  • Diaminobenzidine
  • Immunohistochemistry
  • Marker amplification
  • Marker chemistry
  • Physical developer

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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