The c-ros tyrosine kinase receptor controls regionalization and differentiation of epithelial cells in the epididymis

Eva Sonnenberg-Riethmacher, Barbara Walter, Dieter Riethmacher, Stephanie Gödecke, Carmen Birchmeier

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

The c-ros gene was originally identified in mutant form as an oncogene. The proto-oncogene encodes a tyrosine kinase receptor that is expressed in a small number of epithelial cell types, including those of the epididymis. Targeted mutations of c-ros in the mouse reveal an essential role of the gene in male fertility. Male c-ros -/- animals do not reproduce, whereas the fertility of female animals is not affected. We demonstrate that c-ros is not required in a cell autonomous manner for male germ cell development or function. The gene, therefore, does not affect sperm generation or function in a direct manner. The primary defect in the mutant animals was located in the epididymis, showing that c-ros controls appropriate development of the epithelia, particularly regionalization and terminal differentiation. The epididymal defect does not interfere with production or storage of sperm but, rather, with sperm maturation and the ability of sperm to fertilize in vivo. Interestingly, sperm isolated from c-ros -/- animals can fertilize in vitro. Our results highlight the essential role of the epididymis in male fertility and demonstrate a highly specific function of the c-ros receptor tyrosine kinase during development of distinct epithelial cells.

Original languageEnglish
Pages (from-to)1184-1193
Number of pages10
JournalGenes and Development
Volume10
Issue number10
Publication statusPublished - May 15 1996
Externally publishedYes

Fingerprint

Epididymis
Receptor Protein-Tyrosine Kinases
Spermatozoa
Epithelial Cells
Fertility
Sperm Maturation
Proto-Oncogenes
Essential Genes
Oncogenes
Germ Cells
Genes
Epithelium
Mutation

Keywords

  • c-ros tyrosine kinase receptor
  • epididymis
  • epithelial cells
  • regionalization
  • terminal differentiation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The c-ros tyrosine kinase receptor controls regionalization and differentiation of epithelial cells in the epididymis. / Sonnenberg-Riethmacher, Eva; Walter, Barbara; Riethmacher, Dieter; Gödecke, Stephanie; Birchmeier, Carmen.

In: Genes and Development, Vol. 10, No. 10, 15.05.1996, p. 1184-1193.

Research output: Contribution to journalArticle

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