The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials

A. W. Lloyd, S. Dropcova, R. G A Faragher, P. R. Gard, G. W. Hanlon, S. V. Mikhalovsky, C. J. Olliff, S. P. Denyer, E. Letko, M. Filipec

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recent developments in ocular implant technology require the in vitro evaluation of ocular compatibility in early stage development programs. This requires an understanding and appreciation of the biological interactions which occur in the ocular environment and their relevance with respect to the clinical complications associated with surgical implantation of devices. This paper describes the development of a series of clinically reflective in vitro assays for assessing the potential ocular compatibility of novel intraocular lens materials. Staphylococcus epidermidis attachment, fibrinogen adsorption, mouse embryo fibroblast 3T3 adhesion and proliferation, primary rabbit lens cell adhesion, human peripheral blood macrophage adhesion and granulocyte activation tests were employed to evaluate two widely used intraocular biomaterials poly(methyl methacrylate) (PMMA) and silicone, and a novel biomimetic phosphorylcholine-based coating (PC). The performance of these materials in the in vitro assays was compared to their ability to reduce postoperative inflammation in vivo in a rabbit model. The results demonstrated that the in vitro assays described here are predictive of in vivo ocular compatibility. These assays offer a more relevant means of assessing the ocular compatibility of biomaterials than those presently required by the authorities for regulatory approval of medical devices and implants.

Original languageEnglish
Pages (from-to)621-627
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Volume10
Issue number10-11
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Biocompatible Materials
Biocompatibility
Biomaterials
Assays
Adhesion
Intraocular lenses
Phosphorylcholine
Macrophages
Cell adhesion
Biomimetics
Polymethyl Methacrylate
Silicones
Fibroblasts
Polymethyl methacrylates
Fibrinogen
Rabbits
Lenses
Equipment and Supplies
Blood
Staphylococcus epidermidis

ASJC Scopus subject areas

  • Biophysics
  • Chemical Engineering(all)
  • Bioengineering

Cite this

Lloyd, A. W., Dropcova, S., Faragher, R. G. A., Gard, P. R., Hanlon, G. W., Mikhalovsky, S. V., ... Filipec, M. (1999). The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials. Journal of Materials Science: Materials in Medicine, 10(10-11), 621-627.

The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials. / Lloyd, A. W.; Dropcova, S.; Faragher, R. G A; Gard, P. R.; Hanlon, G. W.; Mikhalovsky, S. V.; Olliff, C. J.; Denyer, S. P.; Letko, E.; Filipec, M.

In: Journal of Materials Science: Materials in Medicine, Vol. 10, No. 10-11, 1999, p. 621-627.

Research output: Contribution to journalArticle

Lloyd, AW, Dropcova, S, Faragher, RGA, Gard, PR, Hanlon, GW, Mikhalovsky, SV, Olliff, CJ, Denyer, SP, Letko, E & Filipec, M 1999, 'The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials', Journal of Materials Science: Materials in Medicine, vol. 10, no. 10-11, pp. 621-627.
Lloyd AW, Dropcova S, Faragher RGA, Gard PR, Hanlon GW, Mikhalovsky SV et al. The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials. Journal of Materials Science: Materials in Medicine. 1999;10(10-11):621-627.
Lloyd, A. W. ; Dropcova, S. ; Faragher, R. G A ; Gard, P. R. ; Hanlon, G. W. ; Mikhalovsky, S. V. ; Olliff, C. J. ; Denyer, S. P. ; Letko, E. ; Filipec, M. / The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials. In: Journal of Materials Science: Materials in Medicine. 1999 ; Vol. 10, No. 10-11. pp. 621-627.
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