The development of multicolor panels with Quantum dots-conjugated antibodies for diagnostics of chronic lymphoid leukemia (CLL) by conventional flow cytometer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

The standard flow cytometry diagnostics approach (3 colors) requires 10-15 tubes per patient and does not allow identification of small populations of tumor cells. Quantum dots (QDots) offer new possibilities in polychromatic clinical cytometry resolving organic and tandem dyes issues with stability and donor bleed-through. We used combinations of beads stained with different antibody conjugates with QDots and standard fluorochromes to calculate signal to noise ratio and stain index for QD605, QD655 and QD705-conjugated antibodies alone and included in our CLL panels. We developed 8-color panel (CD3/CD5/CD19/CD23/CD38/CD10/CD45/Sytox Blue viability dye) panel incorporating QD605, QD655 and QD705 direct antibody conjugates (Invitrogen) for differential diagnostics of CLL. Using this panel we were able to define the population of CLL cells that was as small as 1% of the overall PBMC population - it was determined in dilution experiments (adding mixture of ∼0.2% CLL to the normal blood).

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Pages26-28
Number of pages3
Publication statusPublished - Dec 1 2009
EventNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States
Duration: May 3 2009May 7 2009

Publication series

NameTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Volume2

Other

OtherNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
CountryUnited States
CityHouston, TX
Period5/3/095/7/09

Keywords

  • Antibodies
  • Chronic lymphoid leukemia
  • Flow cytometry
  • Quantum Dots

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials

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