Abstract
Aims: To investigate the effect of different fixation and processing regimens on the assessment of oestrogen receptor (ER) by immunohistochemistry (IHC). Methods: The ER results for 420 patients from seven different hospitals in which antigen retrieval and IHC were performed centrally were compared. The intensity of ER positivity was assessed semiquantitatively using the Quick score (range 0-7). The scoring profiles of cases from each different source (hospital) were compared to detect differences in the proportion of cases that were negative (Quick score = 0), moderately positive (Quick score = 1-5), and strongly positive (Quick score = 6-7). Results: There were no significant differences (p = 0.3; χ2 test) in the proportion of cases in each category. Conclusions: None of the fixation or processing regimens had a significant adverse effect on the sensitivity of the ER assessment performed by automated immunohistochemistry.
| Original language | English |
|---|---|
| Pages (from-to) | 236-238 |
| Number of pages | 3 |
| Journal | Journal of Clinical Pathology |
| Volume | 55 |
| Issue number | 3 |
| Publication status | Published - 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
The effect of fixation and processing on the sensitivity of oestrogen receptor assay by immunohistochemistry in breast carcinoma. / Lee, H.; Douglas-Jones, A. G.; Morgan, J. M.; Jasani, B.
In: Journal of Clinical Pathology, Vol. 55, No. 3, 2002, p. 236-238.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of fixation and processing on the sensitivity of oestrogen receptor assay by immunohistochemistry in breast carcinoma
AU - Lee, H.
AU - Douglas-Jones, A. G.
AU - Morgan, J. M.
AU - Jasani, B.
PY - 2002
Y1 - 2002
N2 - Aims: To investigate the effect of different fixation and processing regimens on the assessment of oestrogen receptor (ER) by immunohistochemistry (IHC). Methods: The ER results for 420 patients from seven different hospitals in which antigen retrieval and IHC were performed centrally were compared. The intensity of ER positivity was assessed semiquantitatively using the Quick score (range 0-7). The scoring profiles of cases from each different source (hospital) were compared to detect differences in the proportion of cases that were negative (Quick score = 0), moderately positive (Quick score = 1-5), and strongly positive (Quick score = 6-7). Results: There were no significant differences (p = 0.3; χ2 test) in the proportion of cases in each category. Conclusions: None of the fixation or processing regimens had a significant adverse effect on the sensitivity of the ER assessment performed by automated immunohistochemistry.
AB - Aims: To investigate the effect of different fixation and processing regimens on the assessment of oestrogen receptor (ER) by immunohistochemistry (IHC). Methods: The ER results for 420 patients from seven different hospitals in which antigen retrieval and IHC were performed centrally were compared. The intensity of ER positivity was assessed semiquantitatively using the Quick score (range 0-7). The scoring profiles of cases from each different source (hospital) were compared to detect differences in the proportion of cases that were negative (Quick score = 0), moderately positive (Quick score = 1-5), and strongly positive (Quick score = 6-7). Results: There were no significant differences (p = 0.3; χ2 test) in the proportion of cases in each category. Conclusions: None of the fixation or processing regimens had a significant adverse effect on the sensitivity of the ER assessment performed by automated immunohistochemistry.
UR - http://www.scopus.com/inward/record.url?scp=0036201456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036201456&partnerID=8YFLogxK
M3 - Article
C2 - 11896082
AN - SCOPUS:0036201456
VL - 55
SP - 236
EP - 238
JO - Journal of Clinical Pathology - Clinical Molecular Pathology
JF - Journal of Clinical Pathology - Clinical Molecular Pathology
SN - 0021-9746
IS - 3
ER -