The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers

I. I. Ismailov, B. K. Berdiev, D. I. Benos

Research output: Contribution to journalArticle

Abstract

A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

lipid bilayers
Hydrostatic Pressure
Lipid bilayers
Lipid Bilayers
Hydrostatic pressure
Pressure gradient
Cations
Permeability
cations
permeability
Membranes
Amiloride
Divalent Cations
colon
Rats
Colon
Chemical activation
calcium
rats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers. / Ismailov, I. I.; Berdiev, B. K.; Benos, D. I.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Ismailov, I. I. ; Berdiev, B. K. ; Benos, D. I. / The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
@article{7292c25cda26464f97a92813c22cd2d9,
title = "The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers",
abstract = "A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.",
author = "Ismailov, {I. I.} and Berdiev, {B. K.} and Benos, {D. I.}",
year = "1996",
language = "English",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers

AU - Ismailov, I. I.

AU - Berdiev, B. K.

AU - Benos, D. I.

PY - 1996

Y1 - 1996

N2 - A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.

AB - A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.

UR - http://www.scopus.com/inward/record.url?scp=33748954259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748954259&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -