Abstract
A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 3 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers. / Ismailov, I. I.; Berdiev, B. K.; Benos, D. I.
In: FASEB Journal, Vol. 10, No. 3, 1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of stretch on the cation permeability properties of ENaC in planar lipid bilayers
AU - Ismailov, I. I.
AU - Berdiev, B. K.
AU - Benos, D. I.
PY - 1996
Y1 - 1996
N2 - A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.
AB - A low conductance, amiloride-sensitive Na+ channel has been cloned from rat distal colon by Canessa al. (Nature (1994) 367:463-467). This channel, termed rENaC, consists of three homologous subunits, a, , and y, and forms single Naselective channels when incorporated into planar lipid bilayers. rENaC can be activated following imposition of a hydrostatic pressure gradient (AP) across the bilayer membrane. When AP = 0, the relative cation permeability sequence (determined from reversal potential measurements made under biionic conditions) of rENaC was: PL|(1.72) > PN>(1.00) > PK(0.11) > > PRb (0.01) - PC, ( PN, (1.00) > PK (0.71) PMg (0.62) > Pc, (0.54) > PRb (0.32) > PC, (0.15) PS, (0.01) = PB, (-rENaC (P0 = 0.65 ±0.06) when AP = 0. Imposition of a hydrostatic pressure gradient under Ca2+-free conditions did not further activate these channels. These results suggest that activation rENaC produced by membrane stretch may be due to release of divalent cation block.
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M3 - Article
VL - 10
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 3
ER -