The effects of temperature and viscosity on glucose diffusivity through Saccharomyces cerevisiae biofilms

Attilio Converti, Mario Zilli, Saleh Arni, Renzo Di Felice, Marco Del Borghi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study helps us to understand the effects of temperature and viscosity on bioprocesses in immobilized cell bioreactors. Glucose diffusivities through Saccharomyces cerevisiae biofilms are experimentally investigated at variable biofilm thickness, temperature, and concentration of carboxymethylcellulose (CMC) used as viscosity-raising additive. Comparing these experimental values with those for free diffusion of glucose in water shows us that biofilm strongly reduces diffusivity (about 74%), mainly due to a decrease in the effective diffusion area. Only a slight increase in difrusivity is observed when biofilm thickness is increased. In addition, the results obtained in this work demonstrate that the well-known empirical equations of Wilke and Chang, and of Guzman and Andrade can be combined to a relationship which takes into account the simultaneous effects of temperature and viscosity on the effective glucose diffusivity. This equation appears to be valid in glucose aqueous solutions whether or not biofilm is used, whereas it is not applicable when viscosity-raising additives, such as CMC, are used.

Original languageEnglish
Pages (from-to)618-626
Number of pages9
JournalCanadian Journal of Chemical Engineering
Volume77
Issue number4
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Biofilms
Yeast
Glucose
Viscosity
Carboxymethylcellulose Sodium
Temperature
Bioreactors
Cells
Water

Keywords

  • Biofilm thickness
  • Diffusivity
  • Glucose
  • Temperature
  • Viscosity

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

The effects of temperature and viscosity on glucose diffusivity through Saccharomyces cerevisiae biofilms. / Converti, Attilio; Zilli, Mario; Arni, Saleh; Di Felice, Renzo; Del Borghi, Marco.

In: Canadian Journal of Chemical Engineering, Vol. 77, No. 4, 1999, p. 618-626.

Research output: Contribution to journalArticle

Converti, A, Zilli, M, Arni, S, Di Felice, R & Del Borghi, M 1999, 'The effects of temperature and viscosity on glucose diffusivity through Saccharomyces cerevisiae biofilms', Canadian Journal of Chemical Engineering, vol. 77, no. 4, pp. 618-626.
Converti, Attilio ; Zilli, Mario ; Arni, Saleh ; Di Felice, Renzo ; Del Borghi, Marco. / The effects of temperature and viscosity on glucose diffusivity through Saccharomyces cerevisiae biofilms. In: Canadian Journal of Chemical Engineering. 1999 ; Vol. 77, No. 4. pp. 618-626.
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