The extracellular-matrix protein matrilin 2 participates in peripheral nerve regeneration

Dmitry Malin, Eva Sonnenberg-Riethmacher, Daria Guseva, Raimund Wagener, Attila Aszódi, Audrey Irintchev, Dieter Riethmacher

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Matrilins are adaptor proteins of the extracellular matrix involved in the formation of both collagen-dependent and collagen-independent filamentous networks. Although their molecular structure and binding partners have been characterized, the functional roles of the four matrilin family members in vivo are still largely unknown. Here, we show that matrilin 2, expressed in pre-myelinating Schwann cells during normal development, profoundly influences the behaviour of glial cells and neurons in vitro. When offered as a uniform substrate, matrilin 2 increased neurite outgrowth of dorsal root ganglia (DRG) neurons and enhanced the migration of both cell line- and embryonic DRG-derived Schwann cells. Vice versa, axonal outgrowth and cell migration were decreased in DRG cultures prepared from matrilin-2-deficient mice compared with wild-type (wt) cultures. In stripe assays, matrilin 2 alone was sufficient to guide axonal growth and, interestingly, axons favoured the combination of matrilin 2 and laminin over laminin alone. In vivo, matrilin 2 was strongly upregulated in injured peripheral nerves of adult wild-type mice and failure of protein upregulation in knockout mice resulted in delayed regrowth of regenerating axons and delayed time-course of functional recovery. Strikingly, the functional recovery 2 months after nerve injury was inferior in matrilin-2-deficient mice compared with wild-type littermates, although motoneuron survival, quality of axonal regeneration, estimated by analyses of axonal diameters and degrees of myelination, and Schwann cell proliferation were not influenced by the mutation. These results show that matrilin 2 is a permissive substrate for axonal growth and cell migration, and that it is required for successful nerve regeneration.

Original languageEnglish
Pages (from-to)995-1004
Number of pages10
JournalJournal of Cell Science
Volume122
Issue number7
DOIs
Publication statusPublished - Apr 1 2009
Externally publishedYes

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Matrilin Proteins
Nerve Regeneration
Extracellular Matrix Proteins
Peripheral Nerves
Schwann Cells
Spinal Ganglia
Laminin
Cell Movement
Axons
Collagen
Neurons
Motor Neurons
Growth
Molecular Structure
Knockout Mice
Neuroglia
Extracellular Matrix
Regeneration
Up-Regulation

Keywords

  • Axonal outgrowth
  • Cell migration
  • Coating substrates
  • Extracellular matrix
  • Peripheral nerve regeneration
  • Schwann cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

The extracellular-matrix protein matrilin 2 participates in peripheral nerve regeneration. / Malin, Dmitry; Sonnenberg-Riethmacher, Eva; Guseva, Daria; Wagener, Raimund; Aszódi, Attila; Irintchev, Audrey; Riethmacher, Dieter.

In: Journal of Cell Science, Vol. 122, No. 7, 01.04.2009, p. 995-1004.

Research output: Contribution to journalArticle

Malin, Dmitry ; Sonnenberg-Riethmacher, Eva ; Guseva, Daria ; Wagener, Raimund ; Aszódi, Attila ; Irintchev, Audrey ; Riethmacher, Dieter. / The extracellular-matrix protein matrilin 2 participates in peripheral nerve regeneration. In: Journal of Cell Science. 2009 ; Vol. 122, No. 7. pp. 995-1004.
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