The fission yeast chromo domain encoding gene chp1(+) is required for chromosome segregation and shows a genetic interaction with alpha-tubulin

C L Doe, G Wang, C Chow, M D Fricker, P B Singh, E J Mellor

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In eukaryotes, the segregation of chromosomes is co-ordinated by the centromere and must proceed accurately if aneuploidy and cell death are to be avoided. The fission yeast centromere is complex, containing highly repetitive regions of DNA showing the characteristics of heterochromatin. Two proteins, Swi6p and Clr4p, that are associated with the fission yeast centromere also contain a chromo (chromatin organisation modifier) domain and are required for centromere function. We have analysed a novel fission yeast gene encoding a putative chromo domain called chp 1(+) (chromo domain protein in Schizosaccharomyces p ombe ). In the absence of Chp1p protein, cells are viable but show chromosome segregation defects such as lagging chromosomes on the spindle during anaphase and high rates of minichromosome loss, phenotypes which are also displayed by swi 6 and clr 4. A fusion protein between green fluorescent protein (GFP) and Chp1p, like Swi6p, is localized to discrete sites within the nucleus. In contrast to Swi6p and Clr4p, Chp1p is not required to repress silent mating-type genes. We demonstrate a genetic interaction between chp 1(+) and alpha-tubulin ( nda 2(+)) and between swi 6(+) and beta-tubulin ( nda 3(+)). Chp1p and Swi6p proteins may be components of the kinetochore which captures and stabilizes the microtubules of the spindle.

Original languageEnglish
Pages (from-to)4222-9
Number of pages8
JournalNucleic Acids Research
Volume26
Issue number18
Publication statusPublished - Sep 15 1998

Fingerprint

Chromosome Segregation
Schizosaccharomyces
Centromere
Tubulin
Chromatin
Genes
Proteins
Kinetochores
Anaphase
Heterochromatin
Nucleic Acid Repetitive Sequences
Aneuploidy
Green Fluorescent Proteins
Eukaryota
Microtubules
Cell Death
Chromosomes
Phenotype
DNA

Keywords

  • Amino Acid Sequence
  • Anaphase
  • Aneuploidy
  • Cell Cycle Proteins
  • Chromosome Segregation
  • Chromosomes, Fungal
  • Fungal Proteins
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Methyltransferases
  • Molecular Sequence Data
  • Mutagenesis
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Spindle Apparatus
  • Transcription Factors
  • Tubulin
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

The fission yeast chromo domain encoding gene chp1(+) is required for chromosome segregation and shows a genetic interaction with alpha-tubulin. / Doe, C L; Wang, G; Chow, C; Fricker, M D; Singh, P B; Mellor, E J.

In: Nucleic Acids Research, Vol. 26, No. 18, 15.09.1998, p. 4222-9.

Research output: Contribution to journalArticle

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abstract = "In eukaryotes, the segregation of chromosomes is co-ordinated by the centromere and must proceed accurately if aneuploidy and cell death are to be avoided. The fission yeast centromere is complex, containing highly repetitive regions of DNA showing the characteristics of heterochromatin. Two proteins, Swi6p and Clr4p, that are associated with the fission yeast centromere also contain a chromo (chromatin organisation modifier) domain and are required for centromere function. We have analysed a novel fission yeast gene encoding a putative chromo domain called chp 1(+) (chromo domain protein in Schizosaccharomyces p ombe ). In the absence of Chp1p protein, cells are viable but show chromosome segregation defects such as lagging chromosomes on the spindle during anaphase and high rates of minichromosome loss, phenotypes which are also displayed by swi 6 and clr 4. A fusion protein between green fluorescent protein (GFP) and Chp1p, like Swi6p, is localized to discrete sites within the nucleus. In contrast to Swi6p and Clr4p, Chp1p is not required to repress silent mating-type genes. We demonstrate a genetic interaction between chp 1(+) and alpha-tubulin ( nda 2(+)) and between swi 6(+) and beta-tubulin ( nda 3(+)). Chp1p and Swi6p proteins may be components of the kinetochore which captures and stabilizes the microtubules of the spindle.",
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T1 - The fission yeast chromo domain encoding gene chp1(+) is required for chromosome segregation and shows a genetic interaction with alpha-tubulin

AU - Doe, C L

AU - Wang, G

AU - Chow, C

AU - Fricker, M D

AU - Singh, P B

AU - Mellor, E J

PY - 1998/9/15

Y1 - 1998/9/15

N2 - In eukaryotes, the segregation of chromosomes is co-ordinated by the centromere and must proceed accurately if aneuploidy and cell death are to be avoided. The fission yeast centromere is complex, containing highly repetitive regions of DNA showing the characteristics of heterochromatin. Two proteins, Swi6p and Clr4p, that are associated with the fission yeast centromere also contain a chromo (chromatin organisation modifier) domain and are required for centromere function. We have analysed a novel fission yeast gene encoding a putative chromo domain called chp 1(+) (chromo domain protein in Schizosaccharomyces p ombe ). In the absence of Chp1p protein, cells are viable but show chromosome segregation defects such as lagging chromosomes on the spindle during anaphase and high rates of minichromosome loss, phenotypes which are also displayed by swi 6 and clr 4. A fusion protein between green fluorescent protein (GFP) and Chp1p, like Swi6p, is localized to discrete sites within the nucleus. In contrast to Swi6p and Clr4p, Chp1p is not required to repress silent mating-type genes. We demonstrate a genetic interaction between chp 1(+) and alpha-tubulin ( nda 2(+)) and between swi 6(+) and beta-tubulin ( nda 3(+)). Chp1p and Swi6p proteins may be components of the kinetochore which captures and stabilizes the microtubules of the spindle.

AB - In eukaryotes, the segregation of chromosomes is co-ordinated by the centromere and must proceed accurately if aneuploidy and cell death are to be avoided. The fission yeast centromere is complex, containing highly repetitive regions of DNA showing the characteristics of heterochromatin. Two proteins, Swi6p and Clr4p, that are associated with the fission yeast centromere also contain a chromo (chromatin organisation modifier) domain and are required for centromere function. We have analysed a novel fission yeast gene encoding a putative chromo domain called chp 1(+) (chromo domain protein in Schizosaccharomyces p ombe ). In the absence of Chp1p protein, cells are viable but show chromosome segregation defects such as lagging chromosomes on the spindle during anaphase and high rates of minichromosome loss, phenotypes which are also displayed by swi 6 and clr 4. A fusion protein between green fluorescent protein (GFP) and Chp1p, like Swi6p, is localized to discrete sites within the nucleus. In contrast to Swi6p and Clr4p, Chp1p is not required to repress silent mating-type genes. We demonstrate a genetic interaction between chp 1(+) and alpha-tubulin ( nda 2(+)) and between swi 6(+) and beta-tubulin ( nda 3(+)). Chp1p and Swi6p proteins may be components of the kinetochore which captures and stabilizes the microtubules of the spindle.

KW - Amino Acid Sequence

KW - Anaphase

KW - Aneuploidy

KW - Cell Cycle Proteins

KW - Chromosome Segregation

KW - Chromosomes, Fungal

KW - Fungal Proteins

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Methyltransferases

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Recombinant Fusion Proteins

KW - Saccharomyces cerevisiae Proteins

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Spindle Apparatus

KW - Transcription Factors

KW - Tubulin

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 26

SP - 4222

EP - 4229

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 18

ER -