The impact of the bystander effect on the low-dose hypersensitivity phenomenon

Otilia Nuta, Firouz Darroudi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The aim of this study was to investigate the possible relationship between the bystander effect and the low-dose hypersensitivity/increased radio-resistance phenomenon in BJ fibroblast cells taking as response criteria different end points of radiation damage such as cell survival, chromosomal damage (as detected by using micronucleus assay) and double strand breaks (DSBs) of the DNA. Although γ-H2AX foci were observed in confluent bystander BJ cells, our data suggest that X-irradiation does not lead to a significant rate of DSBs in bystander cells. Thus, neither bystander effect induced unstable chromosomal aberrations nor bystander effect induced DSBs are sufficiently pronounced to explain the apparent relationship between bystander effect and low-dose hypersensitivity. The experiments described here suggest that the hyper-radiosensitivity phenomenon might be related to bystander factor induced cell inactivation in the low-dose region (lower than 1 Gy).

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalRadiation and Environmental Biophysics
Volume47
Issue number2
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

Fingerprint

Bystander Effect
hypersensitivity
Hypersensitivity
Cells
strands
dosage
Radiation damage
Fibroblasts
Aberrations
Assays
radiation resistance
chromosome aberrations
cells
Irradiation
radiation damage
Micronucleus Tests
cell viability
fibroblasts
Double-Stranded DNA Breaks
Radiation Tolerance

ASJC Scopus subject areas

  • Environmental Science (miscellaneous)
  • Radiation
  • Biophysics
  • Physics and Astronomy (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Environmental Science(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Environmental Chemistry

Cite this

The impact of the bystander effect on the low-dose hypersensitivity phenomenon. / Nuta, Otilia; Darroudi, Firouz.

In: Radiation and Environmental Biophysics, Vol. 47, No. 2, 04.2008, p. 265-274.

Research output: Contribution to journalArticle

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