The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair

Rostislav V. Shevchenko, Marc Eeman, Behzad Rowshanravan, Iain U. Allan, Irina N. Savina, Matt Illsley, Michel Salmon, Stuart L. James, Sergey V. Mikhalovsky, S. Elizabeth James

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131 ± 17 μm at one surface decreasing to 30 ± 8 μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28 day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications.

Original languageEnglish
Pages (from-to)3156-3166
Number of pages11
JournalActa Biomaterialia
Volume10
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Gelatin
Scaffolds
Repair
Wound Healing
Skin
Wounds and Injuries
Silicones
Cryogels
Keratinocytes
Cell Movement
Regeneration
Swine
Fibroblasts
Mechanical stability
Cell Proliferation
Infiltration
Pore size
In Vitro Techniques
Substitution reactions
Elastic moduli

Keywords

  • Cryogel
  • Dermal skin substitute
  • Macroporous scaffold
  • Pig model
  • Wound healing

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Shevchenko, R. V., Eeman, M., Rowshanravan, B., Allan, I. U., Savina, I. N., Illsley, M., ... James, S. E. (2014). The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair. Acta Biomaterialia, 10(7), 3156-3166. https://doi.org/10.1016/j.actbio.2014.03.027

The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair. / Shevchenko, Rostislav V.; Eeman, Marc; Rowshanravan, Behzad; Allan, Iain U.; Savina, Irina N.; Illsley, Matt; Salmon, Michel; James, Stuart L.; Mikhalovsky, Sergey V.; James, S. Elizabeth.

In: Acta Biomaterialia, Vol. 10, No. 7, 2014, p. 3156-3166.

Research output: Contribution to journalArticle

Shevchenko, RV, Eeman, M, Rowshanravan, B, Allan, IU, Savina, IN, Illsley, M, Salmon, M, James, SL, Mikhalovsky, SV & James, SE 2014, 'The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair', Acta Biomaterialia, vol. 10, no. 7, pp. 3156-3166. https://doi.org/10.1016/j.actbio.2014.03.027
Shevchenko, Rostislav V. ; Eeman, Marc ; Rowshanravan, Behzad ; Allan, Iain U. ; Savina, Irina N. ; Illsley, Matt ; Salmon, Michel ; James, Stuart L. ; Mikhalovsky, Sergey V. ; James, S. Elizabeth. / The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 7. pp. 3156-3166.
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