Abstract
A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131 ± 17 μm at one surface decreasing to 30 ± 8 μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28 day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications.
| Original language | English |
|---|---|
| Pages (from-to) | 3156-3166 |
| Number of pages | 11 |
| Journal | Acta Biomaterialia |
| Volume | 10 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Cryogel
- Dermal skin substitute
- Macroporous scaffold
- Pig model
- Wound healing
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- Biotechnology
- Biochemistry
- Molecular Biology
- Medicine(all)
Cite this
The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair. / Shevchenko, Rostislav V.; Eeman, Marc; Rowshanravan, Behzad; Allan, Iain U.; Savina, Irina N.; Illsley, Matt; Salmon, Michel; James, Stuart L.; Mikhalovsky, Sergey V.; James, S. Elizabeth.
In: Acta Biomaterialia, Vol. 10, No. 7, 2014, p. 3156-3166.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair
AU - Shevchenko, Rostislav V.
AU - Eeman, Marc
AU - Rowshanravan, Behzad
AU - Allan, Iain U.
AU - Savina, Irina N.
AU - Illsley, Matt
AU - Salmon, Michel
AU - James, Stuart L.
AU - Mikhalovsky, Sergey V.
AU - James, S. Elizabeth
PY - 2014
Y1 - 2014
N2 - A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131 ± 17 μm at one surface decreasing to 30 ± 8 μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28 day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications.
AB - A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131 ± 17 μm at one surface decreasing to 30 ± 8 μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28 day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications.
KW - Cryogel
KW - Dermal skin substitute
KW - Macroporous scaffold
KW - Pig model
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=84901792360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901792360&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2014.03.027
DO - 10.1016/j.actbio.2014.03.027
M3 - Article
C2 - 24704695
AN - SCOPUS:84901792360
VL - 10
SP - 3156
EP - 3166
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 7
ER -