The in vitro corneal biocompatibility of hydroxyapatite coated carbon mesh

Susan R. Sandeman, Hannah Jeffery, Carol A. Howell, Martin Smith, Sergey V. Mikhalovsky, Andrew W. Lloyd

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of this study was to consider the use of a hydroxyapatite (HA) coated porous carbon matrix as a synthetic dental laminate substitute in osteo-odonto-keratoprosthetic (OOKP) design. 3 types of carbon meshes were coated with HA by sonoelectrochemical deposition. The materials were characterised by scanning electron microscopy (SEM) and HA deposition was characterised by elemental analysis and X-ray diffractometry (XRD). In vitro assays were carried out to quantify the effects of HA coating on human keratocyte adhesion. Cellular cytokine production was used to assess inflammatory potential. HA coating significantly increased keratocyte adhesion to the carbon matrix (p <0.01). The materials did not induce excessive cytokine production by the adherent keratocytes. In addition, the matrices themselves adsorbed significant levels of the cytokine IL-8 (p <0.05). The results indicate that HA coated carbon matrices provide a suitable environment to enhance in-growth of corneal cells without inducing further inflammation. The materials may also suppress excessive inflammation by adsorption of the cytokine IL-8 into the porous, internal carbon structure. Crown

Original languageEnglish
Pages (from-to)3143-3149
Number of pages7
JournalBiomaterials
Volume30
Issue number18
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint

Durapatite
Biocompatibility
Hydroxyapatite
Carbon
Cytokines
Interleukin-8
Dental Veneers
Adhesion
Inflammation
Coatings
Crowns
Electron Scanning Microscopy
X ray diffraction analysis
Adsorption
Laminates
In Vitro Techniques
Assays
Cells
X-Rays
Scanning electron microscopy

Keywords

  • Biocompatibility
  • Carbon
  • Cornea
  • Cytokine
  • Wound healing

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Sandeman, S. R., Jeffery, H., Howell, C. A., Smith, M., Mikhalovsky, S. V., & Lloyd, A. W. (2009). The in vitro corneal biocompatibility of hydroxyapatite coated carbon mesh. Biomaterials, 30(18), 3143-3149. https://doi.org/10.1016/j.biomaterials.2009.02.042

The in vitro corneal biocompatibility of hydroxyapatite coated carbon mesh. / Sandeman, Susan R.; Jeffery, Hannah; Howell, Carol A.; Smith, Martin; Mikhalovsky, Sergey V.; Lloyd, Andrew W.

In: Biomaterials, Vol. 30, No. 18, 06.2009, p. 3143-3149.

Research output: Contribution to journalArticle

Sandeman, SR, Jeffery, H, Howell, CA, Smith, M, Mikhalovsky, SV & Lloyd, AW 2009, 'The in vitro corneal biocompatibility of hydroxyapatite coated carbon mesh', Biomaterials, vol. 30, no. 18, pp. 3143-3149. https://doi.org/10.1016/j.biomaterials.2009.02.042
Sandeman SR, Jeffery H, Howell CA, Smith M, Mikhalovsky SV, Lloyd AW. The in vitro corneal biocompatibility of hydroxyapatite coated carbon mesh. Biomaterials. 2009 Jun;30(18):3143-3149. https://doi.org/10.1016/j.biomaterials.2009.02.042
Sandeman, Susan R. ; Jeffery, Hannah ; Howell, Carol A. ; Smith, Martin ; Mikhalovsky, Sergey V. ; Lloyd, Andrew W. / The in vitro corneal biocompatibility of hydroxyapatite coated carbon mesh. In: Biomaterials. 2009 ; Vol. 30, No. 18. pp. 3143-3149.
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