The inner nuclear membrane protein lamin B receptor forms distinct microdomains and links epigenetically marked chromatin to the nuclear envelope

Dimitra Makatsori, Niki Kourmouli, Hara Polioudaki, Leonard D Shultz, Kelvin McLean, Panayiotis A Theodoropoulos, Prim B Singh, Spyros D Georgatos

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Using heterochromatin-enriched fractions, we have detected specific binding of mononucleosomes to the N-terminal domain of the inner nuclear membrane protein lamin B receptor. Mass spectrometric analysis reveals that LBR-associated particles contain complex patterns of methylated/acetylated histones and are devoid of "euchromatic" epigenetic marks. LBR binds heterochromatin as a higher oligomer and forms distinct nuclear envelope microdomains in vivo. The organization of these membrane assemblies is affected significantly in heterozygous ic (ichthyosis) mutants, resulting in a variety of structural abnormalities and nuclear defects.

Original languageEnglish
Pages (from-to)25567-73
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number24
DOIs
Publication statusPublished - Jun 11 2004

Fingerprint

Heterochromatin
Nuclear Envelope
Nuclear Proteins
Oligomers
Histones
Chromatin
Membrane Proteins
Membranes
Ichthyosis
Defects
Epigenomics
IgA receptor
lamin B receptor

Keywords

  • Animals
  • HeLa Cells
  • Heterochromatin
  • Humans
  • Mass Spectrometry
  • Nuclear Envelope
  • Receptors, Cytoplasmic and Nuclear
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

Cite this

Makatsori, D., Kourmouli, N., Polioudaki, H., Shultz, L. D., McLean, K., Theodoropoulos, P. A., ... Georgatos, S. D. (2004). The inner nuclear membrane protein lamin B receptor forms distinct microdomains and links epigenetically marked chromatin to the nuclear envelope. Journal of Biological Chemistry, 279(24), 25567-73. https://doi.org/10.1074/jbc.M313606200

The inner nuclear membrane protein lamin B receptor forms distinct microdomains and links epigenetically marked chromatin to the nuclear envelope. / Makatsori, Dimitra; Kourmouli, Niki; Polioudaki, Hara; Shultz, Leonard D; McLean, Kelvin; Theodoropoulos, Panayiotis A; Singh, Prim B; Georgatos, Spyros D.

In: Journal of Biological Chemistry, Vol. 279, No. 24, 11.06.2004, p. 25567-73.

Research output: Contribution to journalArticle

Makatsori, Dimitra ; Kourmouli, Niki ; Polioudaki, Hara ; Shultz, Leonard D ; McLean, Kelvin ; Theodoropoulos, Panayiotis A ; Singh, Prim B ; Georgatos, Spyros D. / The inner nuclear membrane protein lamin B receptor forms distinct microdomains and links epigenetically marked chromatin to the nuclear envelope. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 24. pp. 25567-73.
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