Abstract
The expression of the nuclear protein Ki-67 (pKi-67) is strictly correlated with cell proliferation. Because of this, anti-Ki-67 antibodies can be used as operational markers to estimate the growth fraction of human neoplasia in situ. For a variety of tumours, the assessment of pKi-67 expression has repeatedly been proven to be of prognostic value for survival and tumour recurrence, but no cellular function has yet been ascribed to the Ki-67 protein. This study shows that a C-terminal domain of pKi-67 (Kon21) is able to bind to all three members of the mammalian heterochromatin protein 1 (HP1) family in vitro and in vivo. This interaction can be manipulated in living cells, as evidenced by ectopic expression of GFP-tagged HP1 proteins in HeLa cells, which results in a dramatic relocalization of endogenous pKi-67. Taken together, the data presented in this study suggest a role for pKi-67 in the control of higher-order chromatin structure.
| Original language | English |
|---|---|
| Pages (from-to) | 135-44 |
| Number of pages | 10 |
| Journal | Journal of Pathology |
| Volume | 196 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2002 |
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Keywords
- Animals
- Cell Division
- Cell Nucleus
- Chromatin
- Chromosomal Proteins, Non-Histone
- Fluorescent Antibody Technique
- Green Fluorescent Proteins
- HeLa Cells
- Humans
- Interphase
- Ki-67 Antigen
- Luminescent Proteins
- Protein Isoforms
- Recombinant Fusion Proteins
- Transfection
- Two-Hybrid System Techniques
- Journal Article
- Research Support, Non-U.S. Gov't
Cite this
The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family : a potential role in the regulation of higher-order chromatin structure. / Scholzen, Thomas; Endl, Elmar; Wohlenberg, Claudia; van der Sar, Sjaak; Cowell, Ian G; Gerdes, Johannes; Singh, Prim B.
In: Journal of Pathology, Vol. 196, No. 2, 02.2002, p. 135-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family
T2 - a potential role in the regulation of higher-order chromatin structure
AU - Scholzen, Thomas
AU - Endl, Elmar
AU - Wohlenberg, Claudia
AU - van der Sar, Sjaak
AU - Cowell, Ian G
AU - Gerdes, Johannes
AU - Singh, Prim B
N1 - Copyright 2001 John Wiley & Sons, Ltd.
PY - 2002/2
Y1 - 2002/2
N2 - The expression of the nuclear protein Ki-67 (pKi-67) is strictly correlated with cell proliferation. Because of this, anti-Ki-67 antibodies can be used as operational markers to estimate the growth fraction of human neoplasia in situ. For a variety of tumours, the assessment of pKi-67 expression has repeatedly been proven to be of prognostic value for survival and tumour recurrence, but no cellular function has yet been ascribed to the Ki-67 protein. This study shows that a C-terminal domain of pKi-67 (Kon21) is able to bind to all three members of the mammalian heterochromatin protein 1 (HP1) family in vitro and in vivo. This interaction can be manipulated in living cells, as evidenced by ectopic expression of GFP-tagged HP1 proteins in HeLa cells, which results in a dramatic relocalization of endogenous pKi-67. Taken together, the data presented in this study suggest a role for pKi-67 in the control of higher-order chromatin structure.
AB - The expression of the nuclear protein Ki-67 (pKi-67) is strictly correlated with cell proliferation. Because of this, anti-Ki-67 antibodies can be used as operational markers to estimate the growth fraction of human neoplasia in situ. For a variety of tumours, the assessment of pKi-67 expression has repeatedly been proven to be of prognostic value for survival and tumour recurrence, but no cellular function has yet been ascribed to the Ki-67 protein. This study shows that a C-terminal domain of pKi-67 (Kon21) is able to bind to all three members of the mammalian heterochromatin protein 1 (HP1) family in vitro and in vivo. This interaction can be manipulated in living cells, as evidenced by ectopic expression of GFP-tagged HP1 proteins in HeLa cells, which results in a dramatic relocalization of endogenous pKi-67. Taken together, the data presented in this study suggest a role for pKi-67 in the control of higher-order chromatin structure.
KW - Animals
KW - Cell Division
KW - Cell Nucleus
KW - Chromatin
KW - Chromosomal Proteins, Non-Histone
KW - Fluorescent Antibody Technique
KW - Green Fluorescent Proteins
KW - HeLa Cells
KW - Humans
KW - Interphase
KW - Ki-67 Antigen
KW - Luminescent Proteins
KW - Protein Isoforms
KW - Recombinant Fusion Proteins
KW - Transfection
KW - Two-Hybrid System Techniques
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1002/path.1016
DO - 10.1002/path.1016
M3 - Article
VL - 196
SP - 135
EP - 144
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 2
ER -