The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the regulation of higher-order chromatin structure

Thomas Scholzen, Elmar Endl, Claudia Wohlenberg, Sjaak van der Sar, Ian G Cowell, Johannes Gerdes, Prim B Singh

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The expression of the nuclear protein Ki-67 (pKi-67) is strictly correlated with cell proliferation. Because of this, anti-Ki-67 antibodies can be used as operational markers to estimate the growth fraction of human neoplasia in situ. For a variety of tumours, the assessment of pKi-67 expression has repeatedly been proven to be of prognostic value for survival and tumour recurrence, but no cellular function has yet been ascribed to the Ki-67 protein. This study shows that a C-terminal domain of pKi-67 (Kon21) is able to bind to all three members of the mammalian heterochromatin protein 1 (HP1) family in vitro and in vivo. This interaction can be manipulated in living cells, as evidenced by ectopic expression of GFP-tagged HP1 proteins in HeLa cells, which results in a dramatic relocalization of endogenous pKi-67. Taken together, the data presented in this study suggest a role for pKi-67 in the control of higher-order chromatin structure.

Original languageEnglish
Pages (from-to)135-44
Number of pages10
JournalJournal of Pathology
Volume196
Issue number2
DOIs
Publication statusPublished - Feb 2002

Keywords

  • Animals
  • Cell Division
  • Cell Nucleus
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Fluorescent Antibody Technique
  • Green Fluorescent Proteins
  • HeLa Cells
  • Humans
  • Interphase
  • Ki-67 Antigen
  • Luminescent Proteins
  • Protein Isoforms
  • Recombinant Fusion Proteins
  • Transfection
  • Two-Hybrid System Techniques
  • Journal Article
  • Research Support, Non-U.S. Gov't

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