Abstract
Rats were dosed with methylmercuric chloride, either by gastric gavage (5 × 10 mg kg-1 body weight over a 15-day period), or in their drinking water (20 mg methylmercuric chloride l-1 for 14 or 42 days). Localization of mercury within the cerebellum was performed with a silver physical development technique, and metallothionein with dinitrophenyl hapten-sandwich immunohistochemistry. Mercury was detected in structurally undamaged Purkinje neurones and adjacent Bergmann glial cells; no mercury was detected in granule cells even though these small cells nearest the Purkinje layer had a high incidence of pyknotic nuclei. In general, metallothionein was detected mainly in Bergmann glial cells, Purkinje cells, astrocytes and glial cells of white matter; no metallothionein was detected in granule cells. We hypothesized that the resistance of Purkinje cells to methylmercuric chloride reflects their ability to transform organic mercurials to inorganic mercury that, in turn, induces the synthesis of radical-scavenging metallothionein molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 161-169 |
| Number of pages | 9 |
| Journal | The Histochemical Journal |
| Volume | 26 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Anatomy
- Cell Biology
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The localization of mercury and metallothionein in the cerebellum of rats experimentally exposed to methylmercury. / Leyshon-Sørland, K.; Jasani, B.; Morgan, A. J.
In: The Histochemical Journal, Vol. 26, No. 2, 02.1994, p. 161-169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The localization of mercury and metallothionein in the cerebellum of rats experimentally exposed to methylmercury
AU - Leyshon-Sørland, K.
AU - Jasani, B.
AU - Morgan, A. J.
PY - 1994/2
Y1 - 1994/2
N2 - Rats were dosed with methylmercuric chloride, either by gastric gavage (5 × 10 mg kg-1 body weight over a 15-day period), or in their drinking water (20 mg methylmercuric chloride l-1 for 14 or 42 days). Localization of mercury within the cerebellum was performed with a silver physical development technique, and metallothionein with dinitrophenyl hapten-sandwich immunohistochemistry. Mercury was detected in structurally undamaged Purkinje neurones and adjacent Bergmann glial cells; no mercury was detected in granule cells even though these small cells nearest the Purkinje layer had a high incidence of pyknotic nuclei. In general, metallothionein was detected mainly in Bergmann glial cells, Purkinje cells, astrocytes and glial cells of white matter; no metallothionein was detected in granule cells. We hypothesized that the resistance of Purkinje cells to methylmercuric chloride reflects their ability to transform organic mercurials to inorganic mercury that, in turn, induces the synthesis of radical-scavenging metallothionein molecules.
AB - Rats were dosed with methylmercuric chloride, either by gastric gavage (5 × 10 mg kg-1 body weight over a 15-day period), or in their drinking water (20 mg methylmercuric chloride l-1 for 14 or 42 days). Localization of mercury within the cerebellum was performed with a silver physical development technique, and metallothionein with dinitrophenyl hapten-sandwich immunohistochemistry. Mercury was detected in structurally undamaged Purkinje neurones and adjacent Bergmann glial cells; no mercury was detected in granule cells even though these small cells nearest the Purkinje layer had a high incidence of pyknotic nuclei. In general, metallothionein was detected mainly in Bergmann glial cells, Purkinje cells, astrocytes and glial cells of white matter; no metallothionein was detected in granule cells. We hypothesized that the resistance of Purkinje cells to methylmercuric chloride reflects their ability to transform organic mercurials to inorganic mercury that, in turn, induces the synthesis of radical-scavenging metallothionein molecules.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028222750&partnerID=8YFLogxK
U2 - 10.1007/BF00157965
DO - 10.1007/BF00157965
M3 - Article
C2 - 8150662
AN - SCOPUS:0028222750
VL - 26
SP - 161
EP - 169
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 2
ER -