The M31 gene has a complex developmentally regulated expression profile and may encode alternative protein products that possess diverse subcellular localisation patterns

K Peterson, G Wang, D Horsley, J C Richardson, C Sapienza, K E Latham, P B Singh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

HP1-like chromobox genes comprise an evolutionarily conserved family of genes that encode components of centromeric heterochromatin. In order to investigate the role of the murine HP1-like gene, M31, in heterochromatin formation we have isolated its gene and characterised its transcripts and protein products. PCR products that represent M31 transcripts were detected at the one-cell stage and were maternal in origin. Maternal provision of M31 transcripts may reflect a need for M31 in the formation of a functional centromere in order that there is proper segregation of chromosomes during the early cleavage divisions; studies in fission yeast and Drosophila have suggested a crucial role for HP1-like genes in centromere function. There are three protein products encoded by the M31 gene. Surprisingly, the two smaller products are found almost exclusively in the cytoplasm.

Original languageEnglish
Pages (from-to)288-303
Number of pages16
JournalJournal of Experimental Zoology
Volume280
Issue number4
Publication statusPublished - Mar 1 1998

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protein products
genes
centromeres
heterochromatin
chromosome segregation
Schizosaccharomyces pombe
Drosophila
cytoplasm
mice
cells

Keywords

  • Alternative Splicing
  • Animals
  • Blastocyst
  • Centromere
  • Chromosomal Proteins, Non-Histone
  • Cloning, Molecular
  • Cytoplasm
  • DNA, Complementary
  • Exons
  • Gene Expression Regulation, Developmental
  • Genes
  • Heterochromatin
  • Introns
  • Male
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Sequence Analysis, DNA
  • Testis
  • Transcription, Genetic
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

The M31 gene has a complex developmentally regulated expression profile and may encode alternative protein products that possess diverse subcellular localisation patterns. / Peterson, K; Wang, G; Horsley, D; Richardson, J C; Sapienza, C; Latham, K E; Singh, P B.

In: Journal of Experimental Zoology, Vol. 280, No. 4, 01.03.1998, p. 288-303.

Research output: Contribution to journalArticle

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AU - Horsley, D

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AU - Sapienza, C

AU - Latham, K E

AU - Singh, P B

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KW - Alternative Splicing

KW - Animals

KW - Blastocyst

KW - Centromere

KW - Chromosomal Proteins, Non-Histone

KW - Cloning, Molecular

KW - Cytoplasm

KW - DNA, Complementary

KW - Exons

KW - Gene Expression Regulation, Developmental

KW - Genes

KW - Heterochromatin

KW - Introns

KW - Male

KW - Mice

KW - Molecular Sequence Data

KW - RNA, Messenger

KW - Recombinant Fusion Proteins

KW - Sequence Analysis, DNA

KW - Testis

KW - Transcription, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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