The M31 gene has a complex developmentally regulated expression profile and may encode alternative protein products that possess diverse subcellular localisation patterns

K Peterson, G Wang, D Horsley, J C Richardson, C Sapienza, K E Latham, P B Singh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

HP1-like chromobox genes comprise an evolutionarily conserved family of genes that encode components of centromeric heterochromatin. In order to investigate the role of the murine HP1-like gene, M31, in heterochromatin formation we have isolated its gene and characterised its transcripts and protein products. PCR products that represent M31 transcripts were detected at the one-cell stage and were maternal in origin. Maternal provision of M31 transcripts may reflect a need for M31 in the formation of a functional centromere in order that there is proper segregation of chromosomes during the early cleavage divisions; studies in fission yeast and Drosophila have suggested a crucial role for HP1-like genes in centromere function. There are three protein products encoded by the M31 gene. Surprisingly, the two smaller products are found almost exclusively in the cytoplasm.

Original languageEnglish
Pages (from-to)288-303
Number of pages16
JournalJournal of Experimental Zoology
Volume280
Issue number4
Publication statusPublished - Mar 1 1998

Keywords

  • Alternative Splicing
  • Animals
  • Blastocyst
  • Centromere
  • Chromosomal Proteins, Non-Histone
  • Cloning, Molecular
  • Cytoplasm
  • DNA, Complementary
  • Exons
  • Gene Expression Regulation, Developmental
  • Genes
  • Heterochromatin
  • Introns
  • Male
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Sequence Analysis, DNA
  • Testis
  • Transcription, Genetic
  • Journal Article
  • Research Support, Non-U.S. Gov't

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