The murine heterochromatin protein M31 is associated with the chromocenter in round spermatids and Is a component of mature spermatozoa

S Hoyer-Fender, P B Singh, D Motzkus

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

In mature sperm the normal nucleosomal packaging of DNA found in somatic and meiotic cells is transformed into a highly condensed form of chromatin which consists mostly of nucleoprotamines. Although sperm DNA is highly condensed it is nevertheless packaged into a highly defined nuclear architecture which may be organized by the heterochromatic chromocenter. One major component of heterochromatin is the heterochromatin protein 1 which is involved in epigenetic gene silencing. In order to investigate the possible involvement of heterochromatin protein in higher order organization of sperm DNA we studied the localization of the murine homologue of heterochromatin protein 1, M31, during chromatin reorganization in male germ cell differentiation. Each cell type in the testis showed a unique distribution pattern of M31. Colocalization to the heterochromatic regions were found in Sertoli cells, in midstage pachytene spermatocytes, and in round spermatids in which M31 localizes to the centromeric chromocenter. M31 cannot be detected in elongated spermatids or mature spermatozoa immunocytologically, but could be detected in mature spermatozoa by Western blotting. We suggest that M31, a nuclear protein involved in the organization of chromatin architecture, is involved in higher order organization of sperm DNA.

Original languageEnglish
Pages (from-to)72-9
Number of pages8
JournalExperimental Cell Research
Volume254
Issue number1
DOIs
Publication statusPublished - Jan 10 2000

Fingerprint

Spermatids
Heterochromatin
Spermatozoa
Chromatin
Proteins
DNA
DNA Packaging
Spermatocytes
Sertoli Cells
Gene Silencing
Nuclear Proteins
Epigenomics
Germ Cells
Testis
Cell Differentiation
Western Blotting

Keywords

  • Animals
  • Centromere
  • Chromosomal Proteins, Non-Histone
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Rats
  • Spermatids
  • Spermatocytes
  • Spermatogonia
  • Spermatozoa
  • Journal Article

Cite this

The murine heterochromatin protein M31 is associated with the chromocenter in round spermatids and Is a component of mature spermatozoa. / Hoyer-Fender, S; Singh, P B; Motzkus, D.

In: Experimental Cell Research, Vol. 254, No. 1, 10.01.2000, p. 72-9.

Research output: Contribution to journalArticle

@article{7053dceba81e44209a57bdc9fe90190a,
title = "The murine heterochromatin protein M31 is associated with the chromocenter in round spermatids and Is a component of mature spermatozoa",
abstract = "In mature sperm the normal nucleosomal packaging of DNA found in somatic and meiotic cells is transformed into a highly condensed form of chromatin which consists mostly of nucleoprotamines. Although sperm DNA is highly condensed it is nevertheless packaged into a highly defined nuclear architecture which may be organized by the heterochromatic chromocenter. One major component of heterochromatin is the heterochromatin protein 1 which is involved in epigenetic gene silencing. In order to investigate the possible involvement of heterochromatin protein in higher order organization of sperm DNA we studied the localization of the murine homologue of heterochromatin protein 1, M31, during chromatin reorganization in male germ cell differentiation. Each cell type in the testis showed a unique distribution pattern of M31. Colocalization to the heterochromatic regions were found in Sertoli cells, in midstage pachytene spermatocytes, and in round spermatids in which M31 localizes to the centromeric chromocenter. M31 cannot be detected in elongated spermatids or mature spermatozoa immunocytologically, but could be detected in mature spermatozoa by Western blotting. We suggest that M31, a nuclear protein involved in the organization of chromatin architecture, is involved in higher order organization of sperm DNA.",
keywords = "Animals, Centromere, Chromosomal Proteins, Non-Histone, Male, Mice, Mice, Inbred C57BL, Rats, Spermatids, Spermatocytes, Spermatogonia, Spermatozoa, Journal Article",
author = "S Hoyer-Fender and Singh, {P B} and D Motzkus",
note = "Copyright 2000 Academic Press.",
year = "2000",
month = "1",
day = "10",
doi = "10.1006/excr.1999.4729",
language = "English",
volume = "254",
pages = "72--9",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The murine heterochromatin protein M31 is associated with the chromocenter in round spermatids and Is a component of mature spermatozoa

AU - Hoyer-Fender, S

AU - Singh, P B

AU - Motzkus, D

N1 - Copyright 2000 Academic Press.

PY - 2000/1/10

Y1 - 2000/1/10

N2 - In mature sperm the normal nucleosomal packaging of DNA found in somatic and meiotic cells is transformed into a highly condensed form of chromatin which consists mostly of nucleoprotamines. Although sperm DNA is highly condensed it is nevertheless packaged into a highly defined nuclear architecture which may be organized by the heterochromatic chromocenter. One major component of heterochromatin is the heterochromatin protein 1 which is involved in epigenetic gene silencing. In order to investigate the possible involvement of heterochromatin protein in higher order organization of sperm DNA we studied the localization of the murine homologue of heterochromatin protein 1, M31, during chromatin reorganization in male germ cell differentiation. Each cell type in the testis showed a unique distribution pattern of M31. Colocalization to the heterochromatic regions were found in Sertoli cells, in midstage pachytene spermatocytes, and in round spermatids in which M31 localizes to the centromeric chromocenter. M31 cannot be detected in elongated spermatids or mature spermatozoa immunocytologically, but could be detected in mature spermatozoa by Western blotting. We suggest that M31, a nuclear protein involved in the organization of chromatin architecture, is involved in higher order organization of sperm DNA.

AB - In mature sperm the normal nucleosomal packaging of DNA found in somatic and meiotic cells is transformed into a highly condensed form of chromatin which consists mostly of nucleoprotamines. Although sperm DNA is highly condensed it is nevertheless packaged into a highly defined nuclear architecture which may be organized by the heterochromatic chromocenter. One major component of heterochromatin is the heterochromatin protein 1 which is involved in epigenetic gene silencing. In order to investigate the possible involvement of heterochromatin protein in higher order organization of sperm DNA we studied the localization of the murine homologue of heterochromatin protein 1, M31, during chromatin reorganization in male germ cell differentiation. Each cell type in the testis showed a unique distribution pattern of M31. Colocalization to the heterochromatic regions were found in Sertoli cells, in midstage pachytene spermatocytes, and in round spermatids in which M31 localizes to the centromeric chromocenter. M31 cannot be detected in elongated spermatids or mature spermatozoa immunocytologically, but could be detected in mature spermatozoa by Western blotting. We suggest that M31, a nuclear protein involved in the organization of chromatin architecture, is involved in higher order organization of sperm DNA.

KW - Animals

KW - Centromere

KW - Chromosomal Proteins, Non-Histone

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Rats

KW - Spermatids

KW - Spermatocytes

KW - Spermatogonia

KW - Spermatozoa

KW - Journal Article

U2 - 10.1006/excr.1999.4729

DO - 10.1006/excr.1999.4729

M3 - Article

VL - 254

SP - 72

EP - 79

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -