TY - JOUR
T1 - The ParaSight®-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria
AU - Di Perri, Giovanni
AU - Olliaro, Piero
AU - Nardi, Stefano
AU - Allegranzi, Benedetta
AU - Deganello, Roberto
AU - Vento, Sandro
AU - Lanzafame, Massimiliano
AU - Cazzadori, Angelo
AU - Bonora, Stefano
AU - Concia, Ercole
PY - 1997/1/1
Y1 - 1997/1/1
N2 - Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts ≤ 100/μL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/μL and 0.458 and 0.966 respectively for counts ≤ 100/μL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.
AB - Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts ≤ 100/μL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/μL and 0.458 and 0.966 respectively for counts ≤ 100/μL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.
KW - Diagnosis
KW - Dipstick antigen capture assay
KW - Malaria
KW - ParaSight®-F test
KW - Plasmodium falciparum
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U2 - 10.1016/S0035-9203(97)90257-1
DO - 10.1016/S0035-9203(97)90257-1
M3 - Article
C2 - 9373633
AN - SCOPUS:0030613805
VL - 91
SP - 403
EP - 405
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
SN - 0035-9203
IS - 4
ER -