The ParaSight®-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria

Giovanni Di Perri, Piero Olliaro, Stefano Nardi, Benedetta Allegranzi, Roberto Deganello, Sandro Vento, Massimiliano Lanzafame, Angelo Cazzadori, Stefano Bonora, Ercole Concia

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts ≤ 100/μL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/μL and 0.458 and 0.966 respectively for counts ≤ 100/μL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.

Original languageEnglish
Pages (from-to)403-405
Number of pages3
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume91
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Falciparum Malaria
Parasites
Antigens
Pharmaceutical Preparations
Parasitemia
Therapeutics
Antimalarials
Motion Pictures
Plasmodium falciparum
Leukocyte Count
Malaria

Keywords

  • Diagnosis
  • Dipstick antigen capture assay
  • Malaria
  • ParaSight®-F test
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)

Cite this

The ParaSight®-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria. / Di Perri, Giovanni; Olliaro, Piero; Nardi, Stefano; Allegranzi, Benedetta; Deganello, Roberto; Vento, Sandro; Lanzafame, Massimiliano; Cazzadori, Angelo; Bonora, Stefano; Concia, Ercole.

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 91, No. 4, 1997, p. 403-405.

Research output: Contribution to journalArticle

Di Perri, Giovanni ; Olliaro, Piero ; Nardi, Stefano ; Allegranzi, Benedetta ; Deganello, Roberto ; Vento, Sandro ; Lanzafame, Massimiliano ; Cazzadori, Angelo ; Bonora, Stefano ; Concia, Ercole. / The ParaSight®-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria. In: Transactions of the Royal Society of Tropical Medicine and Hygiene. 1997 ; Vol. 91, No. 4. pp. 403-405.
@article{559a1fbd715e4af28323f60dac62d5de,
title = "The ParaSight{\circledR}-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria",
abstract = "Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts ≤ 100/μL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/μL and 0.458 and 0.966 respectively for counts ≤ 100/μL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.",
keywords = "Diagnosis, Dipstick antigen capture assay, Malaria, ParaSight{\circledR}-F test, Plasmodium falciparum",
author = "{Di Perri}, Giovanni and Piero Olliaro and Stefano Nardi and Benedetta Allegranzi and Roberto Deganello and Sandro Vento and Massimiliano Lanzafame and Angelo Cazzadori and Stefano Bonora and Ercole Concia",
year = "1997",
doi = "10.1016/S0035-9203(97)90257-1",
language = "English",
volume = "91",
pages = "403--405",
journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",
issn = "0035-9203",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - The ParaSight®-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria

AU - Di Perri, Giovanni

AU - Olliaro, Piero

AU - Nardi, Stefano

AU - Allegranzi, Benedetta

AU - Deganello, Roberto

AU - Vento, Sandro

AU - Lanzafame, Massimiliano

AU - Cazzadori, Angelo

AU - Bonora, Stefano

AU - Concia, Ercole

PY - 1997

Y1 - 1997

N2 - Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts ≤ 100/μL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/μL and 0.458 and 0.966 respectively for counts ≤ 100/μL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.

AB - Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts ≤ 100/μL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/μL and 0.458 and 0.966 respectively for counts ≤ 100/μL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.

KW - Diagnosis

KW - Dipstick antigen capture assay

KW - Malaria

KW - ParaSight®-F test

KW - Plasmodium falciparum

UR - http://www.scopus.com/inward/record.url?scp=0030613805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030613805&partnerID=8YFLogxK

U2 - 10.1016/S0035-9203(97)90257-1

DO - 10.1016/S0035-9203(97)90257-1

M3 - Article

VL - 91

SP - 403

EP - 405

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

SN - 0035-9203

IS - 4

ER -