The patch-clamp and planar lipid bilayer techniques

Powerful and versatile tools to investigate the CFTR Cl- channel

David N. Sheppard, Michael A. Gray, Xiandi Gong, Yoshiro Sohma, Ilana Kogan, Dale J. Benos, Toby S. Scott-Ward, Jeng Haur Chen, Hongyu Li, Zhiwei Cai, Jyoti Gupta, Canhui Li, Mohabir Ramjeesingh, Bakhrom K. Berdiev, Iskander I. Ismailov, Christine E. Bear, Tzyh Chang Hwang, Paul Linsdell, Martin J. Hug

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalJournal of Cystic Fibrosis
Volume3
Issue numberSUPPL. 2
DOIs
Publication statusPublished - Aug 2004
Externally publishedYes

Fingerprint

Cystic Fibrosis Transmembrane Conductance Regulator
Lipid Bilayers
Patch-Clamp Techniques
Proteins

Keywords

  • Anion permeation
  • Channel gating
  • Channel regulation
  • Chloride channel activity
  • Single-channel recording
  • Whole-cell recording

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Sheppard, D. N., Gray, M. A., Gong, X., Sohma, Y., Kogan, I., Benos, D. J., ... Hug, M. J. (2004). The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel. Journal of Cystic Fibrosis, 3(SUPPL. 2), 101-108. https://doi.org/10.1016/j.jcf.2004.05.046

The patch-clamp and planar lipid bilayer techniques : Powerful and versatile tools to investigate the CFTR Cl- channel. / Sheppard, David N.; Gray, Michael A.; Gong, Xiandi; Sohma, Yoshiro; Kogan, Ilana; Benos, Dale J.; Scott-Ward, Toby S.; Chen, Jeng Haur; Li, Hongyu; Cai, Zhiwei; Gupta, Jyoti; Li, Canhui; Ramjeesingh, Mohabir; Berdiev, Bakhrom K.; Ismailov, Iskander I.; Bear, Christine E.; Hwang, Tzyh Chang; Linsdell, Paul; Hug, Martin J.

In: Journal of Cystic Fibrosis, Vol. 3, No. SUPPL. 2, 08.2004, p. 101-108.

Research output: Contribution to journalArticle

Sheppard, DN, Gray, MA, Gong, X, Sohma, Y, Kogan, I, Benos, DJ, Scott-Ward, TS, Chen, JH, Li, H, Cai, Z, Gupta, J, Li, C, Ramjeesingh, M, Berdiev, BK, Ismailov, II, Bear, CE, Hwang, TC, Linsdell, P & Hug, MJ 2004, 'The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel', Journal of Cystic Fibrosis, vol. 3, no. SUPPL. 2, pp. 101-108. https://doi.org/10.1016/j.jcf.2004.05.046
Sheppard, David N. ; Gray, Michael A. ; Gong, Xiandi ; Sohma, Yoshiro ; Kogan, Ilana ; Benos, Dale J. ; Scott-Ward, Toby S. ; Chen, Jeng Haur ; Li, Hongyu ; Cai, Zhiwei ; Gupta, Jyoti ; Li, Canhui ; Ramjeesingh, Mohabir ; Berdiev, Bakhrom K. ; Ismailov, Iskander I. ; Bear, Christine E. ; Hwang, Tzyh Chang ; Linsdell, Paul ; Hug, Martin J. / The patch-clamp and planar lipid bilayer techniques : Powerful and versatile tools to investigate the CFTR Cl- channel. In: Journal of Cystic Fibrosis. 2004 ; Vol. 3, No. SUPPL. 2. pp. 101-108.
@article{2e78678a6cd141b0bdaaa2afbef036ab,
title = "The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel",
abstract = "Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.",
keywords = "Anion permeation, Channel gating, Channel regulation, Chloride channel activity, Single-channel recording, Whole-cell recording",
author = "Sheppard, {David N.} and Gray, {Michael A.} and Xiandi Gong and Yoshiro Sohma and Ilana Kogan and Benos, {Dale J.} and Scott-Ward, {Toby S.} and Chen, {Jeng Haur} and Hongyu Li and Zhiwei Cai and Jyoti Gupta and Canhui Li and Mohabir Ramjeesingh and Berdiev, {Bakhrom K.} and Ismailov, {Iskander I.} and Bear, {Christine E.} and Hwang, {Tzyh Chang} and Paul Linsdell and Hug, {Martin J.}",
year = "2004",
month = "8",
doi = "10.1016/j.jcf.2004.05.046",
language = "English",
volume = "3",
pages = "101--108",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier",
number = "SUPPL. 2",

}

TY - JOUR

T1 - The patch-clamp and planar lipid bilayer techniques

T2 - Powerful and versatile tools to investigate the CFTR Cl- channel

AU - Sheppard, David N.

AU - Gray, Michael A.

AU - Gong, Xiandi

AU - Sohma, Yoshiro

AU - Kogan, Ilana

AU - Benos, Dale J.

AU - Scott-Ward, Toby S.

AU - Chen, Jeng Haur

AU - Li, Hongyu

AU - Cai, Zhiwei

AU - Gupta, Jyoti

AU - Li, Canhui

AU - Ramjeesingh, Mohabir

AU - Berdiev, Bakhrom K.

AU - Ismailov, Iskander I.

AU - Bear, Christine E.

AU - Hwang, Tzyh Chang

AU - Linsdell, Paul

AU - Hug, Martin J.

PY - 2004/8

Y1 - 2004/8

N2 - Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.

AB - Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.

KW - Anion permeation

KW - Channel gating

KW - Channel regulation

KW - Chloride channel activity

KW - Single-channel recording

KW - Whole-cell recording

UR - http://www.scopus.com/inward/record.url?scp=19944414292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944414292&partnerID=8YFLogxK

U2 - 10.1016/j.jcf.2004.05.046

DO - 10.1016/j.jcf.2004.05.046

M3 - Article

VL - 3

SP - 101

EP - 108

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - SUPPL. 2

ER -