The stochastic model of F1-ATPase molecular motor functioning

Alexandra F. Pogrebnaya, Yury M. Romanovsky, Alexander N. Tikhonov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In continuation of1,2, this work is devoted to the study of the energy characteristics of the F1ATPase-substrate complex. The results of calculations of the electrostatic energy in the enzyme-substrate complex are presented in the first part In calculations, we take into account the electrostatic interactions between the charged groups of the substrate (MgATP) and reaction products (MgADP and Pi) and charged amino acid residues of the α3β3γ complex that correspond to various conformations of the enzyme. The hydrolysis of ATP in the catalytic site leads to coordinated conformational changes in α, β subunits and to ordered rotation of γ subunit located in the center of F1ATPase complex. The calculations show that the energetically favorable process involving MgATP binding at the catalytic site in the "open" conformation initiates γ subunit rotation followed by the hydrolysis in the other (tight) catalytic site. In the second part, we propose the simplest stochastic model describing the ordered rotation of γ subunit (the rotor of F1-ATPase molecular motor). In the model we take into account the electrostatic interaction using the results of the previous calculations. We employ experimentally obtained dynamic parameters from3-6. The model takes into account the thermal fluctuations of the bath and the random processes of the substrate binding and the escape of the reaction products.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsV.V. Tuchin
Pages120-131
Number of pages12
Volume5330
DOIs
Publication statusPublished - 2004
Externally publishedYes
EventComplex Dynamics, Fluctuations, Chaos, and Fractals in Biomedical Photonics - San Jose, CA, United States
Duration: Jan 25 2004Jan 25 2004

Other

OtherComplex Dynamics, Fluctuations, Chaos, and Fractals in Biomedical Photonics
CountryUnited States
CitySan Jose, CA
Period1/25/041/25/04

Keywords

  • ATP hydrolysis
  • Electrostatic energy
  • F-ATPase
  • Mathematical model of molecular motor
  • Molecular motor
  • Stochastic model of molecular motor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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