TY - JOUR
T1 - The Trajectory of Successful Aging
T2 - Insights from Metagenome and Cytokine Profiling
AU - Chulenbayeva, Laura
AU - Ganzhula, Yuliya
AU - Kozhakhmetov, Samat
AU - Jarmukhanov, Zharkyn
AU - Nurgaziyev, Madiyar
AU - Nurgozhina, Ayaulym
AU - Muhanbetzhanov, Nurislam
AU - Sergazy, Shynggys
AU - Zhetkenev, Sanzhar
AU - Borykbay, Zhanar
AU - Tkachev, Viktor
AU - Urazova, Saltanat
AU - Vinogradova, Elizaveta
AU - Kushugulova, Almagul
N1 - Publisher Copyright:
© 2024 The Author(s).
PY - 2024/1/19
Y1 - 2024/1/19
N2 - Introduction: The longevity is influenced by genetic, environmental, and lifestyle factors. The specific changes that occur in the gut microbiome during the aging process, and their relationship to longevity and immune function, have not yet been fully understood. The ongoing research of other microbiome based on longevity cohort in Kazakhstan provides preliminary information on longevity-related aging, where cytokine expression is associated with specific microbial communities and microbial functions. Methods: Metagenomic shotgun sequencing study of 40 long-lived individuals aged 90 years and over was carried out, who were conditionally healthy and active, able to serve themselves, without a history of serious infection and cancer, who had not taken any antimicrobials, including probiotics. Blood serum was analyzed for clinical and laboratory characteristics. The cytokine and chemokine profile in serum and stool samples was assessed using multiplex analysis. Results: We found a significant increase in the expression of proinflammatory cytokines IL-1a, IL-6, 12p70, IP-10, IFNá2, IL- 15, TNFa, as well as chemokines MIP-1a/CCL3 and MIP-1b/ CCL4, chemokine motif ligands MCP-3/CCL7 and MDC/ CCL22(1c). Nonagenerians and centenarians demonstrated a greater diversity of core microbiota genera and showed an elevated prevalence of the genera Bacteroides, Clostridium, Escherichia, and Alistipes. Conversely, there was a decrease in the abundance of the genera Ruminococcus, Fusicatenibacter, Dorea, as well as the species Fusicatenibacter saccharivorans. Furthermore, functional analysis revealed that the microbiome in long-lived group has a high capacity for lipid metabolism, amino acid degradation, and potential signs of chronic inflammatory status. Conclusion: Long-lived individuals exhibit an immune system imbalance and observed changes in the composition of the gut microbiota at the genus level between to the two age-groups. Age-related changes in the gut microbiome, metabolic functions of the microbial community, and chronic inflammation all contribute to immunosenescence. In turn, the inflammatory state and microbial composition of the gut is related to nutritional status.
AB - Introduction: The longevity is influenced by genetic, environmental, and lifestyle factors. The specific changes that occur in the gut microbiome during the aging process, and their relationship to longevity and immune function, have not yet been fully understood. The ongoing research of other microbiome based on longevity cohort in Kazakhstan provides preliminary information on longevity-related aging, where cytokine expression is associated with specific microbial communities and microbial functions. Methods: Metagenomic shotgun sequencing study of 40 long-lived individuals aged 90 years and over was carried out, who were conditionally healthy and active, able to serve themselves, without a history of serious infection and cancer, who had not taken any antimicrobials, including probiotics. Blood serum was analyzed for clinical and laboratory characteristics. The cytokine and chemokine profile in serum and stool samples was assessed using multiplex analysis. Results: We found a significant increase in the expression of proinflammatory cytokines IL-1a, IL-6, 12p70, IP-10, IFNá2, IL- 15, TNFa, as well as chemokines MIP-1a/CCL3 and MIP-1b/ CCL4, chemokine motif ligands MCP-3/CCL7 and MDC/ CCL22(1c). Nonagenerians and centenarians demonstrated a greater diversity of core microbiota genera and showed an elevated prevalence of the genera Bacteroides, Clostridium, Escherichia, and Alistipes. Conversely, there was a decrease in the abundance of the genera Ruminococcus, Fusicatenibacter, Dorea, as well as the species Fusicatenibacter saccharivorans. Furthermore, functional analysis revealed that the microbiome in long-lived group has a high capacity for lipid metabolism, amino acid degradation, and potential signs of chronic inflammatory status. Conclusion: Long-lived individuals exhibit an immune system imbalance and observed changes in the composition of the gut microbiota at the genus level between to the two age-groups. Age-related changes in the gut microbiome, metabolic functions of the microbial community, and chronic inflammation all contribute to immunosenescence. In turn, the inflammatory state and microbial composition of the gut is related to nutritional status.
KW - Aging
KW - Cytokines
KW - Gut microbiome
KW - Long-lived individuals
KW - Longevity
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UR - http://www.scopus.com/inward/citedby.url?scp=85185801787&partnerID=8YFLogxK
U2 - 10.1159/000536082
DO - 10.1159/000536082
M3 - Article
C2 - 38246133
AN - SCOPUS:85185801787
SN - 0304-324X
VL - 70
SP - 390
EP - 407
JO - Gerontology
JF - Gerontology
IS - 4
ER -