The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p

Bianka L. Grosshans, Anna Andreeva, Akanksha Gangar, Sherry Niessen, John R. Yates, Patrick Brennwald, Peter Novick

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Rab guanosine triphosphatases regulate intracellular membrane traffic by binding specific effector proteins. The yeast Rab Sec4p plays multiple roles in the polarized transport of post-Golgi vesicles to, and their subsequent fusion with, the plasma membrane, suggesting the involvement of several effectors. Yet, only one Sec4p effector has been documented to date: the exocyst protein Sec15p. The exocyst is an octameric protein complex required for tethering secretory vesicles, which is a prerequisite for membrane fusion. In this study, we describe the identification of a second Sec4p effector, Sro7p, which is a member of the lethal giant larvae tumor suppressor family. Sec4-GTP binds to Sro7p in cell extracts as well as to purified Sro7p, and the two proteins can be coimmunoprecipitated. Furthermore, we demonstrate the formation of a ternary complex of Sec4-GTP, Sro7p, and the t-SNARE Sec9p. Genetic data support our conclusion that Sro7p functions downstream of Sec4p and further imply that Sro7p and the exocyst share partially overlapping functions, possibly in SNARE regulation.

Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalJournal of Cell Biology
Volume172
Issue number1
DOIs
Publication statusPublished - Feb 1 2006
Externally publishedYes

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rab GTP-Binding Proteins
Yeasts
SNARE Proteins
Guanosine Triphosphate
Proteins
Intracellular Membranes
Membrane Fusion
Guanosine
Secretory Vesicles
Cell Extracts
Larva
Cell Membrane
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Grosshans, B. L., Andreeva, A., Gangar, A., Niessen, S., Yates, J. R., Brennwald, P., & Novick, P. (2006). The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. Journal of Cell Biology, 172(1), 55-66. https://doi.org/10.1083/jcb.200510016

The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. / Grosshans, Bianka L.; Andreeva, Anna; Gangar, Akanksha; Niessen, Sherry; Yates, John R.; Brennwald, Patrick; Novick, Peter.

In: Journal of Cell Biology, Vol. 172, No. 1, 01.02.2006, p. 55-66.

Research output: Contribution to journalArticle

Grosshans, BL, Andreeva, A, Gangar, A, Niessen, S, Yates, JR, Brennwald, P & Novick, P 2006, 'The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p', Journal of Cell Biology, vol. 172, no. 1, pp. 55-66. https://doi.org/10.1083/jcb.200510016
Grosshans, Bianka L. ; Andreeva, Anna ; Gangar, Akanksha ; Niessen, Sherry ; Yates, John R. ; Brennwald, Patrick ; Novick, Peter. / The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. In: Journal of Cell Biology. 2006 ; Vol. 172, No. 1. pp. 55-66.
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