TiO 2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes

Ronghui Wang, Chuanmin Ruan, Damira Kanayeva, Kentu Lassiter, Yanbin Li

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

A novel TiO 2 nanowire bundle microelectrode based immunosensor was demonstrated as a more sensitive, specific, and rapid technology for detection of Listeria monocytogenes. TiO 2 nanowire bundle was prepared through a hydrothermal reaction of alkali with TiO 2 powder and connected to gold microelectrodes with mask welding. Monoclonal antibodies were immobilized on the surface of a TiO 2 nanowire bundle to specifically capture L. monocytogenes. Impedance change caused by the nanowire-antibody-bacteria complex was measured and correlated to bacterial number. This nanowire bundle based immunosensor could detect as low as 10 2 cfu/ml of L. monocytogenes in 1 h without significant interference from other foodborne pathogens.

Original languageEnglish
Pages (from-to)2625-2631
Number of pages7
JournalNano Letters
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Fingerprint

Listeria
Immunosensors
Microelectrodes
bundles
Nanowires
nanowires
impedance
antibodies
Monoclonal antibodies
pathogens
Alkalies
Pathogens
welding
Antibodies
Gold
Powders
bacteria
Masks
alkalies
Bacteria

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

TiO 2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes. / Wang, Ronghui; Ruan, Chuanmin; Kanayeva, Damira; Lassiter, Kentu; Li, Yanbin.

In: Nano Letters, Vol. 8, No. 9, 09.2008, p. 2625-2631.

Research output: Contribution to journalArticle

Wang, Ronghui ; Ruan, Chuanmin ; Kanayeva, Damira ; Lassiter, Kentu ; Li, Yanbin. / TiO 2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes. In: Nano Letters. 2008 ; Vol. 8, No. 9. pp. 2625-2631.
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