Abstract
The mechanisms by which oral tolerance are induced appear to be varied and the ability to tolerize antigen-experienced, or "memory", cells is questionable. An adoptive transfer model was established to permit in vivo characterization of antigen-specific CD4+ T cells in animals before, during, and after tolerization and subsequent antigenic challenge. CD4+ T cells were isolated from DO10 mice bearing an ovalabumin (OVA)-specific transgenic ab-TCR. Naive cells or cells manipulated in vitro to express distinct effector phenotypes were transferred into BALB/c recipients. The recipients were fed ovalbumin in their drinking water for one week and then challenged with OVA intraperitoneally. Mice fed ovalbumin in their drinking water produced ovalbumin-specific serum IgA, and following challenge with OVA, ovalbumin-fed mice produced significantly less OVA-specific serum antibodies than non-fed controls. Use of this model allows the identification of antigen-specific CD4+ T cells in vivo for examination of cytokine production, anatomic localization, and expression of cell surface molecules in a tolerizing environment.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 6 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Tolerization of ovalbumin-specific αβ-tcr transgenic cells in adoptively transferred mice by the feeding of ovalbumin. / Weaver, C. T.; Saparov, A.; Marwill, J. S.; Elson, C. O.; Buoy, R. P.; Kraus, L. A.
In: FASEB Journal, Vol. 10, No. 6, 1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tolerization of ovalbumin-specific αβ-tcr transgenic cells in adoptively transferred mice by the feeding of ovalbumin
AU - Weaver, C. T.
AU - Saparov, A.
AU - Marwill, J. S.
AU - Elson, C. O.
AU - Buoy, R. P.
AU - Kraus, L. A.
PY - 1996
Y1 - 1996
N2 - The mechanisms by which oral tolerance are induced appear to be varied and the ability to tolerize antigen-experienced, or "memory", cells is questionable. An adoptive transfer model was established to permit in vivo characterization of antigen-specific CD4+ T cells in animals before, during, and after tolerization and subsequent antigenic challenge. CD4+ T cells were isolated from DO10 mice bearing an ovalabumin (OVA)-specific transgenic ab-TCR. Naive cells or cells manipulated in vitro to express distinct effector phenotypes were transferred into BALB/c recipients. The recipients were fed ovalbumin in their drinking water for one week and then challenged with OVA intraperitoneally. Mice fed ovalbumin in their drinking water produced ovalbumin-specific serum IgA, and following challenge with OVA, ovalbumin-fed mice produced significantly less OVA-specific serum antibodies than non-fed controls. Use of this model allows the identification of antigen-specific CD4+ T cells in vivo for examination of cytokine production, anatomic localization, and expression of cell surface molecules in a tolerizing environment.
AB - The mechanisms by which oral tolerance are induced appear to be varied and the ability to tolerize antigen-experienced, or "memory", cells is questionable. An adoptive transfer model was established to permit in vivo characterization of antigen-specific CD4+ T cells in animals before, during, and after tolerization and subsequent antigenic challenge. CD4+ T cells were isolated from DO10 mice bearing an ovalabumin (OVA)-specific transgenic ab-TCR. Naive cells or cells manipulated in vitro to express distinct effector phenotypes were transferred into BALB/c recipients. The recipients were fed ovalbumin in their drinking water for one week and then challenged with OVA intraperitoneally. Mice fed ovalbumin in their drinking water produced ovalbumin-specific serum IgA, and following challenge with OVA, ovalbumin-fed mice produced significantly less OVA-specific serum antibodies than non-fed controls. Use of this model allows the identification of antigen-specific CD4+ T cells in vivo for examination of cytokine production, anatomic localization, and expression of cell surface molecules in a tolerizing environment.
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M3 - Article
VL - 10
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 6
ER -