Tolerization of ovalbumin-specific αβ-tcr transgenic cells in adoptively transferred mice by the feeding of ovalbumin

C. T. Weaver, A. Saparov, J. S. Marwill, C. O. Elson, R. P. Buoy, L. A. Kraus

Research output: Contribution to journalArticle

Abstract

The mechanisms by which oral tolerance are induced appear to be varied and the ability to tolerize antigen-experienced, or "memory", cells is questionable. An adoptive transfer model was established to permit in vivo characterization of antigen-specific CD4+ T cells in animals before, during, and after tolerization and subsequent antigenic challenge. CD4+ T cells were isolated from DO10 mice bearing an ovalabumin (OVA)-specific transgenic ab-TCR. Naive cells or cells manipulated in vitro to express distinct effector phenotypes were transferred into BALB/c recipients. The recipients were fed ovalbumin in their drinking water for one week and then challenged with OVA intraperitoneally. Mice fed ovalbumin in their drinking water produced ovalbumin-specific serum IgA, and following challenge with OVA, ovalbumin-fed mice produced significantly less OVA-specific serum antibodies than non-fed controls. Use of this model allows the identification of antigen-specific CD4+ T cells in vivo for examination of cytokine production, anatomic localization, and expression of cell surface molecules in a tolerizing environment.

Original languageEnglish
Pages (from-to)A1192
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - Dec 1 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Weaver, C. T., Saparov, A., Marwill, J. S., Elson, C. O., Buoy, R. P., & Kraus, L. A. (1996). Tolerization of ovalbumin-specific αβ-tcr transgenic cells in adoptively transferred mice by the feeding of ovalbumin. FASEB Journal, 10(6), A1192.