TOR2 is required for organization of the actin cytoskeleton in yeast

Anja Schmidt, Jeannette Kunz, Michael N. Hall

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae gene TOR2 encodes a putative phosphatidylinositol kinase that has two essential functions. One function is redundant with TOR1, a TOR2 homolog, and is required for signaling translation initiation and early G1 progression. The second essential function is unique to TOR2. Here we report that loss of the TOR2-unique function disrupts polarized distribution of the actin cytoskeleton. A screen for dosage suppressors of a dominant negative TOR2 allele identified TCP20/CCT6, encoding a subunit of the cytosolic chaperonin TCP-1 that is involved in the biogenesis of actin structures. Overexpression of TCP20 restores growth and polarized distribution of the actin cytoskeleton in a tor2 mutant. TCP20 overexpression does not restore growth in a tor1 tor2 double mutant. We suggest that the unique function of the phosphatidylinositol kinase homolog TOR2 is required for signaling organization of the actin cytoskeleton during the cell cycle. TOR2, via its two functions, may thus integrate temporal and spatial control of cell growth.

Original languageEnglish
Pages (from-to)13780-13785
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number24
DOIs
Publication statusPublished - Nov 26 1996
Externally publishedYes

Fingerprint

Actin Cytoskeleton
Yeasts
Phosphatidylinositols
Chaperonin Containing TCP-1
Phosphotransferases
Growth
Saccharomyces cerevisiae
Actins
Cell Cycle
Alleles
Genes

Keywords

  • cell growth
  • phosphatidylinositol kinase
  • rapamycin
  • signal transduction
  • TCP-1 chaperonin

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

TOR2 is required for organization of the actin cytoskeleton in yeast. / Schmidt, Anja; Kunz, Jeannette; Hall, Michael N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 24, 26.11.1996, p. 13780-13785.

Research output: Contribution to journalArticle

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