Abstract
We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
Original language | English |
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Pages (from-to) | 231-236 |
Number of pages | 6 |
Journal | Molecular Biology Reports |
Volume | 25 |
Issue number | 4 |
DOIs | |
Publication status | Published - Nov 1998 |
Keywords
- Hepatitis B virus
- Inducible expression system
- Transcriptional activation
- X protein
ASJC Scopus subject areas
- Molecular Biology
- Genetics