Transactivation by expression of the hepatitis B virus X protein with an inducible system

Azliyati Azizan, Yu Sheng Cong, Ken Shain, Shih Chang Tsai, Ya Li Yao, Nancy Olashaw, Edward Seto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalMolecular Biology Reports
Volume25
Issue number4
DOIs
Publication statusPublished - Nov 1998

Keywords

  • Hepatitis B virus
  • Inducible expression system
  • Transcriptional activation
  • X protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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