Transactivation by expression of the hepatitis B virus X protein with an inducible system

Azliyati Azizan; Yu Sheng Cong; Ken Shain; Shih Chang Tsai; Ya Li Yao; Nancy Olashaw; Edward Seto

doi:10.1023/A:1006933629329

Transactivation by expression of the hepatitis B virus X protein with an inducible system

Azliyati Azizan, Yu Sheng Cong, Ken Shain, Shih Chang Tsai, Ya Li Yao, Nancy Olashaw, Edward Seto

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.

Original language	English
Pages (from-to)	231-236
Number of pages	6
Journal	Molecular Biology Reports
Volume	25
Issue number	4
DOIs	https://doi.org/10.1023/A:1006933629329
Publication status	Published - Nov 1998

Keywords

Hepatitis B virus
Inducible expression system
Transcriptional activation
X protein

ASJC Scopus subject areas

Molecular Biology
Genetics

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title = "Transactivation by expression of the hepatitis B virus X protein with an inducible system",

abstract = "We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.",

keywords = "Hepatitis B virus, Inducible expression system, Transcriptional activation, X protein",

author = "Azliyati Azizan and Cong, {Yu Sheng} and Ken Shain and Tsai, {Shih Chang} and Yao, {Ya Li} and Nancy Olashaw and Edward Seto",

note = "Funding Information: We thank Bei Shan for the generous gift of plasmids, Rosalind Jackson for critical reading of the manuscript, and Ourania Andrisani for discussion. This work was supported by grants from the American Cancer Society and by the National Science Foundation (MCB9631067) to E.S.",

year = "1998",

month = nov,

doi = "10.1023/A:1006933629329",

language = "English",

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T1 - Transactivation by expression of the hepatitis B virus X protein with an inducible system

AU - Azizan, Azliyati

AU - Cong, Yu Sheng

AU - Shain, Ken

AU - Tsai, Shih Chang

AU - Yao, Ya Li

AU - Olashaw, Nancy

AU - Seto, Edward

N1 - Funding Information: We thank Bei Shan for the generous gift of plasmids, Rosalind Jackson for critical reading of the manuscript, and Ourania Andrisani for discussion. This work was supported by grants from the American Cancer Society and by the National Science Foundation (MCB9631067) to E.S.

PY - 1998/11

Y1 - 1998/11

N2 - We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.

AB - We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.

KW - Hepatitis B virus

KW - Inducible expression system

KW - Transcriptional activation

KW - X protein

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