Abstract
We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 231-236 |
| Number of pages | 6 |
| Journal | Molecular Biology Reports |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Nov 1998 |
| Externally published | Yes |
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Keywords
- Hepatitis B virus
- Inducible expression system
- Transcriptional activation
- X protein
ASJC Scopus subject areas
- Genetics
- Cell Biology
- Biochemistry
Cite this
Transactivation by expression of the hepatitis B virus X protein with an inducible system. / Azizan, Azliyati; Cong, Yu Sheng; Shain, Ken; Tsai, Shih Chang; Yao, Ya Li; Olashaw, Nancy; Seto, Edward.
In: Molecular Biology Reports, Vol. 25, No. 4, 11.1998, p. 231-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transactivation by expression of the hepatitis B virus X protein with an inducible system
AU - Azizan, Azliyati
AU - Cong, Yu Sheng
AU - Shain, Ken
AU - Tsai, Shih Chang
AU - Yao, Ya Li
AU - Olashaw, Nancy
AU - Seto, Edward
PY - 1998/11
Y1 - 1998/11
N2 - We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
AB - We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
KW - Hepatitis B virus
KW - Inducible expression system
KW - Transcriptional activation
KW - X protein
UR - http://www.scopus.com/inward/record.url?scp=0032212059&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032212059&partnerID=8YFLogxK
U2 - 10.1023/A:1006933629329
DO - 10.1023/A:1006933629329
M3 - Article
VL - 25
SP - 231
EP - 236
JO - Molecular Biology Reports
JF - Molecular Biology Reports
SN - 0301-4851
IS - 4
ER -