Transactivation by expression of the hepatitis B virus X protein with an inducible system

Azliyati Azizan, Yu Sheng Cong, Ken Shain, Shih Chang Tsai, Ya Li Yao, Nancy Olashaw, Edward Seto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalMolecular Biology Reports
Volume25
Issue number4
DOIs
Publication statusPublished - Nov 1998
Externally publishedYes

Fingerprint

Transcriptional Activation
Virus Diseases
Transcription
Fibroblasts
Rats
Carcinogenesis
Proteins
Genes
Cells
Cell Line
DNA
hepatitis B virus X protein

Keywords

  • Hepatitis B virus
  • Inducible expression system
  • Transcriptional activation
  • X protein

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

Transactivation by expression of the hepatitis B virus X protein with an inducible system. / Azizan, Azliyati; Cong, Yu Sheng; Shain, Ken; Tsai, Shih Chang; Yao, Ya Li; Olashaw, Nancy; Seto, Edward.

In: Molecular Biology Reports, Vol. 25, No. 4, 11.1998, p. 231-236.

Research output: Contribution to journalArticle

Azizan, Azliyati ; Cong, Yu Sheng ; Shain, Ken ; Tsai, Shih Chang ; Yao, Ya Li ; Olashaw, Nancy ; Seto, Edward. / Transactivation by expression of the hepatitis B virus X protein with an inducible system. In: Molecular Biology Reports. 1998 ; Vol. 25, No. 4. pp. 231-236.
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