TY - JOUR
T1 - Transcriptional regulation of the mts1 gene in human lymphoma cells
T2 - the role of DNA-methylation
AU - Tulchinsky, Eugene
AU - Grigorian, Mariam
AU - Tkatch, Tatyana
AU - Georgiev, Georgii
AU - Lukanidin, Eugene
PY - 1995/4/4
Y1 - 1995/4/4
N2 - The transcription of the mts1 gene putatively involved in the control of tumor metastasis was studied in three human lymphoma cell lines: MOLT-4, CEM and Jurkat. The level of the mts1 gene transcription is high in MOLT-4 cells, lower in CEM cells and hardly detectable in Jurkat cells. This correlates with the hypomethylation of DNA in the first exon and the first intron of the mts1 gene in the analyzed culture cells. This area was also found to be undermethylated in human peripheral blood cells - macrophages, neutrophils and lymphocytes where the mts1 gene is highly expressed. 5-Azadeoxycytidine (AzadC) - an inhibitor of the eukaryotic DNA-methylase - significantly induces the expression of the mts1 gene in CEM and Jurkat cells and has little effect on mts1 gene transcription in MOLT-4 cells. The drug does not influence mts1 transcription in cultivated peripheral blood lymphocytes. These data indicate the possible involvement of the methylation of the first exon/first intron sequences in the transcriptional repression of the mts1 gene. The finding of two DNAaseI hypersensitivity sites (DHSs) mapped in the first intron of the mts1 gene supports this suggestion.
AB - The transcription of the mts1 gene putatively involved in the control of tumor metastasis was studied in three human lymphoma cell lines: MOLT-4, CEM and Jurkat. The level of the mts1 gene transcription is high in MOLT-4 cells, lower in CEM cells and hardly detectable in Jurkat cells. This correlates with the hypomethylation of DNA in the first exon and the first intron of the mts1 gene in the analyzed culture cells. This area was also found to be undermethylated in human peripheral blood cells - macrophages, neutrophils and lymphocytes where the mts1 gene is highly expressed. 5-Azadeoxycytidine (AzadC) - an inhibitor of the eukaryotic DNA-methylase - significantly induces the expression of the mts1 gene in CEM and Jurkat cells and has little effect on mts1 gene transcription in MOLT-4 cells. The drug does not influence mts1 transcription in cultivated peripheral blood lymphocytes. These data indicate the possible involvement of the methylation of the first exon/first intron sequences in the transcriptional repression of the mts1 gene. The finding of two DNAaseI hypersensitivity sites (DHSs) mapped in the first intron of the mts1 gene supports this suggestion.
KW - DNA methylation
KW - DNAaseI hypersensitivity site
KW - Gene transcription
KW - mts1 gene
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U2 - 10.1016/0167-4781(95)00013-7
DO - 10.1016/0167-4781(95)00013-7
M3 - Article
C2 - 7536040
AN - SCOPUS:0028916485
VL - 1261
SP - 243
EP - 248
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2
ER -