Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa

Hisashi Tamaru, Xing Zhang, Debra McMillen, Prim B Singh, Jun-ichi Nakayama, Shiv I Grewal, C David Allis, Xiaodong Cheng, Eric U Selker

Research output: Contribution to journalArticle

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Abstract

Besides serving to package nuclear DNA, histones carry information in the form of a diverse array of post-translational modifications. Methylation of histones H3 and H4 has been implicated in long-term epigenetic 'memory'. Dimethylation or trimethylation of Lys4 of histone H3 (H3 Lys4) has been found in expressible euchromatin of yeasts and mammals. In contrast, methylation of Lys9 of histone H3 (H3 Lys9) has been implicated in establishing and maintaining the largely quiescent heterochromatin of mammals, yeasts, Drosophila melanogaster and plants. We have previously shown that a DNA methylation mutant of Neurospora crassa, dim-5 (defective in methylation), has a nonsense mutation in the SET domain of an H3-specific histone methyltransferase and that substitutions of H3 Lys9 cause gross hypomethylation of DNA. Similarly, the KRYPTONITE histone methyltransferase is required for full DNA methylation in Arabidopsis thaliana. We used biochemical, genetic and immunological methods to investigate the specific mark for DNA methylation in N. crassa. Here we show that trimethylated H3 Lys9, but not dimethylated H3 Lys9, marks chromatin regions for cytosine methylation and that DIM-5 specifically creates this mark.

Original languageEnglish
Pages (from-to)75-9
Number of pages5
JournalNature Genetics
Volume34
Issue number1
DOIs
Publication statusPublished - May 2003

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Neurospora crassa
DNA Methylation
Histones
Lysine
Methylation
Mammals
Yeasts
Euchromatin
Long-Term Memory
Heterochromatin
Nonsense Codon
Cytosine
DNA
Post Translational Protein Processing
Drosophila melanogaster
Arabidopsis
Epigenomics
Chromatin
Molecular Biology
histone methyltransferase

Keywords

  • DNA Methylation
  • DNA, Fungal
  • Fungal Proteins
  • Genes, Fungal
  • Histone-Lysine N-Methyltransferase
  • Histones
  • Lysine
  • Methylation
  • Methyltransferases
  • Mutation
  • Neurospora crassa
  • Protein Methyltransferases
  • Protein Processing, Post-Translational
  • Journal Article
  • Research Support, U.S. Gov't, P.H.S.

Cite this

Tamaru, H., Zhang, X., McMillen, D., Singh, P. B., Nakayama, J., Grewal, S. I., ... Selker, E. U. (2003). Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa. Nature Genetics, 34(1), 75-9. https://doi.org/10.1038/ng1143

Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa. / Tamaru, Hisashi; Zhang, Xing; McMillen, Debra; Singh, Prim B; Nakayama, Jun-ichi; Grewal, Shiv I; Allis, C David; Cheng, Xiaodong; Selker, Eric U.

In: Nature Genetics, Vol. 34, No. 1, 05.2003, p. 75-9.

Research output: Contribution to journalArticle

Tamaru, H, Zhang, X, McMillen, D, Singh, PB, Nakayama, J, Grewal, SI, Allis, CD, Cheng, X & Selker, EU 2003, 'Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa', Nature Genetics, vol. 34, no. 1, pp. 75-9. https://doi.org/10.1038/ng1143
Tamaru, Hisashi ; Zhang, Xing ; McMillen, Debra ; Singh, Prim B ; Nakayama, Jun-ichi ; Grewal, Shiv I ; Allis, C David ; Cheng, Xiaodong ; Selker, Eric U. / Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa. In: Nature Genetics. 2003 ; Vol. 34, No. 1. pp. 75-9.
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