Abstract
Tuberculosis (TB) remains a major public health concern in most low-income countries. Hence, rapid and sensitive TB diagnostics play an important role in detecting and preventing the disease. In addition to established diagnostic methods, several new approaches have been reported. Some techniques are simple but time-consuming, while others require complex instrumentation. One prominent and readily available approach is to detect proteins that Mycobacterium tuberculosis secretes, such as Mpt64, the 6-kDa early secreted antigenic target (Esat6), the 10-kDa culture filtrate protein (Cfp10), and the antigen 85 (Ag85) complex. Although their functions are not fully understood, a growing body of molecular evidence implicates them in M. tuberculosis virulence. Currently these biomarkers are either being used or investigated for use in skin patch tests, biosensor analyses, and immunochromatographic, immunohistochemical, polymerase chain reaction-based, and enzyme-linked immunosorbent assays. This review provides a comprehensive discussion of the roles these immunodominant antigens play in M. tuberculosis pathogenesis and compares diagnostic methods based on the detection of these proteins with more established tests for TB.
| Original language | English |
|---|---|
| Pages (from-to) | 381-388 |
| Number of pages | 8 |
| Journal | Tuberculosis |
| Volume | 93 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jul 2013 |
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Keywords
- Tuberculosis diagnosis Mpt64 Esat6 Cfp10 Ag85 Biosensor
ASJC Scopus subject areas
- Microbiology
- Immunology
- Infectious Diseases
- Microbiology (medical)
Cite this
Tuberculosis diagnosis using immunodominant, secreted antigens of Mycobacterium tuberculosis. / Bekmurzayeva, Aliya; Sypabekova, Marzhan; Kanayeva, Damira.
In: Tuberculosis, Vol. 93, No. 4, 07.2013, p. 381-388.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tuberculosis diagnosis using immunodominant, secreted antigens of Mycobacterium tuberculosis
AU - Bekmurzayeva, Aliya
AU - Sypabekova, Marzhan
AU - Kanayeva, Damira
PY - 2013/7
Y1 - 2013/7
N2 - Tuberculosis (TB) remains a major public health concern in most low-income countries. Hence, rapid and sensitive TB diagnostics play an important role in detecting and preventing the disease. In addition to established diagnostic methods, several new approaches have been reported. Some techniques are simple but time-consuming, while others require complex instrumentation. One prominent and readily available approach is to detect proteins that Mycobacterium tuberculosis secretes, such as Mpt64, the 6-kDa early secreted antigenic target (Esat6), the 10-kDa culture filtrate protein (Cfp10), and the antigen 85 (Ag85) complex. Although their functions are not fully understood, a growing body of molecular evidence implicates them in M. tuberculosis virulence. Currently these biomarkers are either being used or investigated for use in skin patch tests, biosensor analyses, and immunochromatographic, immunohistochemical, polymerase chain reaction-based, and enzyme-linked immunosorbent assays. This review provides a comprehensive discussion of the roles these immunodominant antigens play in M. tuberculosis pathogenesis and compares diagnostic methods based on the detection of these proteins with more established tests for TB.
AB - Tuberculosis (TB) remains a major public health concern in most low-income countries. Hence, rapid and sensitive TB diagnostics play an important role in detecting and preventing the disease. In addition to established diagnostic methods, several new approaches have been reported. Some techniques are simple but time-consuming, while others require complex instrumentation. One prominent and readily available approach is to detect proteins that Mycobacterium tuberculosis secretes, such as Mpt64, the 6-kDa early secreted antigenic target (Esat6), the 10-kDa culture filtrate protein (Cfp10), and the antigen 85 (Ag85) complex. Although their functions are not fully understood, a growing body of molecular evidence implicates them in M. tuberculosis virulence. Currently these biomarkers are either being used or investigated for use in skin patch tests, biosensor analyses, and immunochromatographic, immunohistochemical, polymerase chain reaction-based, and enzyme-linked immunosorbent assays. This review provides a comprehensive discussion of the roles these immunodominant antigens play in M. tuberculosis pathogenesis and compares diagnostic methods based on the detection of these proteins with more established tests for TB.
KW - Tuberculosis diagnosis Mpt64 Esat6 Cfp10 Ag85 Biosensor
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U2 - 10.1016/j.tube.2013.03.003
DO - 10.1016/j.tube.2013.03.003
M3 - Article
C2 - 23602700
AN - SCOPUS:84878945554
VL - 93
SP - 381
EP - 388
JO - Tuberculosis
JF - Tuberculosis
SN - 1472-9792
IS - 4
ER -