Tysnd1 Deficiency in Mice Interferes with the Peroxisomal Localization of PTS2 Enzymes, Causing Lipid Metabolic Abnormalities and Male Infertility

Yumi Mizuno, Yuichi Ninomiya, Yutaka Nakachi, Mioko Iseki, Hiroyasu Iwasa, Masumi Akita, Tohru Tsukui, Nobuyuki Shimozawa, Chizuru Ito, Kiyotaka Toshimori, Megumi Nishimukai, Hiroshi Hara, Ryouta Maeba, Tomoki Okazaki, Ali Nasser Ali Alodaib, Mohammed Al Amoudi, Minnie Jacob, Fowzan S. Alkuraya, Yasushi Horai, Mitsuhiro WatanabeHiromi Motegi, Shigeharu Wakana, Tetsuo Noda, Igor V. Kurochkin, Yosuke Mizuno, Christian Schönbach, Yasushi Okazaki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Peroxisomes are subcellular organelles involved in lipid metabolic processes, including those of very-long-chain fatty acids and branched-chain fatty acids, among others. Peroxisome matrix proteins are synthesized in the cytoplasm. Targeting signals (PTS or peroxisomal targeting signal) at the C-terminus (PTS1) or N-terminus (PTS2) of peroxisomal matrix proteins mediate their import into the organelle. In the case of PTS2-containing proteins, the PTS2 signal is cleaved from the protein when transported into peroxisomes. The functional mechanism of PTS2 processing, however, is poorly understood. Previously we identified Tysnd1 (Trypsin domain containing 1) and biochemically characterized it as a peroxisomal cysteine endopeptidase that directly processes PTS2-containing prethiolase Acaa1 and PTS1-containing Acox1, Hsd17b4, and ScpX. The latter three enzymes are crucial components of the very-long-chain fatty acids β-oxidation pathway. To clarify the in vivo functions and physiological role of Tysnd1, we analyzed the phenotype of Tysnd1-/- mice. Male Tysnd1-/- mice are infertile, and the epididymal sperms lack the acrosomal cap. These phenotypic features are most likely the result of changes in the molecular species composition of choline and ethanolamine plasmalogens. Tysnd1-/- mice also developed liver dysfunctions when the phytanic acid precursor phytol was orally administered. Phyh and Agps are known PTS2-containing proteins, but were identified as novel Tysnd1 substrates. Loss of Tysnd1 interferes with the peroxisomal localization of Acaa1, Phyh, and Agps, which might cause the mild Zellweger syndrome spectrum-resembling phenotypes. Our data established that peroxisomal processing protease Tysnd1 is necessary to mediate the physiological functions of PTS2-containing substrates.

Original languageEnglish
Article numbere1003286
JournalPLoS Genetics
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

Fingerprint

infertility
Male Infertility
male fertility
abnormality
Trypsin
trypsin
lipid
enzyme
Lipids
protein
mice
Enzymes
lipids
enzymes
fatty acid
Peroxisomes
peroxisomes
targeting
phenotype
very long chain fatty acids

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Tysnd1 Deficiency in Mice Interferes with the Peroxisomal Localization of PTS2 Enzymes, Causing Lipid Metabolic Abnormalities and Male Infertility. / Mizuno, Yumi; Ninomiya, Yuichi; Nakachi, Yutaka; Iseki, Mioko; Iwasa, Hiroyasu; Akita, Masumi; Tsukui, Tohru; Shimozawa, Nobuyuki; Ito, Chizuru; Toshimori, Kiyotaka; Nishimukai, Megumi; Hara, Hiroshi; Maeba, Ryouta; Okazaki, Tomoki; Alodaib, Ali Nasser Ali; Amoudi, Mohammed Al; Jacob, Minnie; Alkuraya, Fowzan S.; Horai, Yasushi; Watanabe, Mitsuhiro; Motegi, Hiromi; Wakana, Shigeharu; Noda, Tetsuo; Kurochkin, Igor V.; Mizuno, Yosuke; Schönbach, Christian; Okazaki, Yasushi.

In: PLoS Genetics, Vol. 9, No. 2, e1003286, 02.2013.

Research output: Contribution to journalArticle

Mizuno, Y, Ninomiya, Y, Nakachi, Y, Iseki, M, Iwasa, H, Akita, M, Tsukui, T, Shimozawa, N, Ito, C, Toshimori, K, Nishimukai, M, Hara, H, Maeba, R, Okazaki, T, Alodaib, ANA, Amoudi, MA, Jacob, M, Alkuraya, FS, Horai, Y, Watanabe, M, Motegi, H, Wakana, S, Noda, T, Kurochkin, IV, Mizuno, Y, Schönbach, C & Okazaki, Y 2013, 'Tysnd1 Deficiency in Mice Interferes with the Peroxisomal Localization of PTS2 Enzymes, Causing Lipid Metabolic Abnormalities and Male Infertility', PLoS Genetics, vol. 9, no. 2, e1003286. https://doi.org/10.1371/journal.pgen.1003286
Mizuno, Yumi ; Ninomiya, Yuichi ; Nakachi, Yutaka ; Iseki, Mioko ; Iwasa, Hiroyasu ; Akita, Masumi ; Tsukui, Tohru ; Shimozawa, Nobuyuki ; Ito, Chizuru ; Toshimori, Kiyotaka ; Nishimukai, Megumi ; Hara, Hiroshi ; Maeba, Ryouta ; Okazaki, Tomoki ; Alodaib, Ali Nasser Ali ; Amoudi, Mohammed Al ; Jacob, Minnie ; Alkuraya, Fowzan S. ; Horai, Yasushi ; Watanabe, Mitsuhiro ; Motegi, Hiromi ; Wakana, Shigeharu ; Noda, Tetsuo ; Kurochkin, Igor V. ; Mizuno, Yosuke ; Schönbach, Christian ; Okazaki, Yasushi. / Tysnd1 Deficiency in Mice Interferes with the Peroxisomal Localization of PTS2 Enzymes, Causing Lipid Metabolic Abnormalities and Male Infertility. In: PLoS Genetics. 2013 ; Vol. 9, No. 2.
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abstract = "Peroxisomes are subcellular organelles involved in lipid metabolic processes, including those of very-long-chain fatty acids and branched-chain fatty acids, among others. Peroxisome matrix proteins are synthesized in the cytoplasm. Targeting signals (PTS or peroxisomal targeting signal) at the C-terminus (PTS1) or N-terminus (PTS2) of peroxisomal matrix proteins mediate their import into the organelle. In the case of PTS2-containing proteins, the PTS2 signal is cleaved from the protein when transported into peroxisomes. The functional mechanism of PTS2 processing, however, is poorly understood. Previously we identified Tysnd1 (Trypsin domain containing 1) and biochemically characterized it as a peroxisomal cysteine endopeptidase that directly processes PTS2-containing prethiolase Acaa1 and PTS1-containing Acox1, Hsd17b4, and ScpX. The latter three enzymes are crucial components of the very-long-chain fatty acids β-oxidation pathway. To clarify the in vivo functions and physiological role of Tysnd1, we analyzed the phenotype of Tysnd1-/- mice. Male Tysnd1-/- mice are infertile, and the epididymal sperms lack the acrosomal cap. These phenotypic features are most likely the result of changes in the molecular species composition of choline and ethanolamine plasmalogens. Tysnd1-/- mice also developed liver dysfunctions when the phytanic acid precursor phytol was orally administered. Phyh and Agps are known PTS2-containing proteins, but were identified as novel Tysnd1 substrates. Loss of Tysnd1 interferes with the peroxisomal localization of Acaa1, Phyh, and Agps, which might cause the mild Zellweger syndrome spectrum-resembling phenotypes. Our data established that peroxisomal processing protease Tysnd1 is necessary to mediate the physiological functions of PTS2-containing substrates.",
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AU - Mizuno, Yumi

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AU - Nakachi, Yutaka

AU - Iseki, Mioko

AU - Iwasa, Hiroyasu

AU - Akita, Masumi

AU - Tsukui, Tohru

AU - Shimozawa, Nobuyuki

AU - Ito, Chizuru

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AU - Maeba, Ryouta

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AU - Alodaib, Ali Nasser Ali

AU - Amoudi, Mohammed Al

AU - Jacob, Minnie

AU - Alkuraya, Fowzan S.

AU - Horai, Yasushi

AU - Watanabe, Mitsuhiro

AU - Motegi, Hiromi

AU - Wakana, Shigeharu

AU - Noda, Tetsuo

AU - Kurochkin, Igor V.

AU - Mizuno, Yosuke

AU - Schönbach, Christian

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