Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

S. E. Burastero, C. Paolucci, D. Breda, G. Monasterolo, R. E. Rossi, L. Vangelista

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Original languageEnglish
Pages (from-to)420-422
Number of pages3
JournalClinical and Vaccine Immunology
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Fingerprint

Immunosorbents
Basophils
Leukotrienes
Assays
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Cell-Free System
Enzymes
Allergens
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit. / Burastero, S. E.; Paolucci, C.; Breda, D.; Monasterolo, G.; Rossi, R. E.; Vangelista, L.

In: Clinical and Vaccine Immunology, Vol. 13, No. 3, 03.2006, p. 420-422.

Research output: Contribution to journalArticle

Burastero, S. E. ; Paolucci, C. ; Breda, D. ; Monasterolo, G. ; Rossi, R. E. ; Vangelista, L. / Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit. In: Clinical and Vaccine Immunology. 2006 ; Vol. 13, No. 3. pp. 420-422.
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