Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

S. E. Burastero, C. Paolucci, D. Breda, G. Monasterolo, R. E. Rossi, L. Vangelista

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Original languageEnglish
Pages (from-to)420-422
Number of pages3
JournalClinical and Vaccine Immunology
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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