TY - JOUR
T1 - Ureides are accumulated similarly in response to UV-C irradiation and wounding in Arabidopsis leaves but are remobilized differently during recovery
AU - Soltabayeva, Aigerim
AU - Bekturova, Aizat
AU - Kurmanbayeva, Assylay
AU - Oshanova, Dinara
AU - Nurbekova, Zhadyrassyn
AU - Srivastava, Sudhakar
AU - Standing, Dominic
AU - Sagi, Moshe
N1 - Funding Information:
This research was supported by the Israel Center of Research Excellence (ICORE) ‘Plant Adaptation’ (ISF grant no. 757/12) and the Gerda Frieberg Chair in Agriculture Water Management.
Publisher Copyright:
© 2022 Oxford University Press. All rights reserved.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Purine degradation products have been shown to play roles in plant response to stresses such as drought, salinity, extended dark, nitrogen deficiency, and pathogen infection. In this study, we used Arabidopsis wild-type (WT) and an Atxdh1-knockout mutant defective in xanthine dehydrogenase1 (XDH1) to examine the role of degraded purine metabolites in the responses to wounding or UV-C stress applied to the middle leaves of the plant. Wounding or UV-C stress in the mutant resulted in lower fresh-weight, increased senescence symptoms, and increased cell death compared to WT plants. In addition, WT plants exhibited lower levels of oxidative stress indicators, reactive oxygen species, and malondialdehyde in their leaves than the mutant. Notably, transcripts and proteins functioning in the purine degradation pathway were regulated in such a way that it led to enhanced ureide levels in WT leaves 24 h after applying the UV-C or wound stress. However, different remobilization of the accumulated ureides was observed after 72 h of stress. In plants treated with UV-C, the concentration of allantoin was highest in young leaves, whereas in wounded plants it was lowest in these leaves and instead accumulated mainly in the middle leaves that had been wounded. These results indicated that in WT plants treated with UV-C, ureides were remobilized from the lower older and damaged leaves to support young leaf growth during the recovery period from stress. After wounding, however, whilst some ureides were remobilized to the young leaves, more remained in the wounded middle leaves to function as antioxidants and/or healing agents.
AB - Purine degradation products have been shown to play roles in plant response to stresses such as drought, salinity, extended dark, nitrogen deficiency, and pathogen infection. In this study, we used Arabidopsis wild-type (WT) and an Atxdh1-knockout mutant defective in xanthine dehydrogenase1 (XDH1) to examine the role of degraded purine metabolites in the responses to wounding or UV-C stress applied to the middle leaves of the plant. Wounding or UV-C stress in the mutant resulted in lower fresh-weight, increased senescence symptoms, and increased cell death compared to WT plants. In addition, WT plants exhibited lower levels of oxidative stress indicators, reactive oxygen species, and malondialdehyde in their leaves than the mutant. Notably, transcripts and proteins functioning in the purine degradation pathway were regulated in such a way that it led to enhanced ureide levels in WT leaves 24 h after applying the UV-C or wound stress. However, different remobilization of the accumulated ureides was observed after 72 h of stress. In plants treated with UV-C, the concentration of allantoin was highest in young leaves, whereas in wounded plants it was lowest in these leaves and instead accumulated mainly in the middle leaves that had been wounded. These results indicated that in WT plants treated with UV-C, ureides were remobilized from the lower older and damaged leaves to support young leaf growth during the recovery period from stress. After wounding, however, whilst some ureides were remobilized to the young leaves, more remained in the wounded middle leaves to function as antioxidants and/or healing agents.
KW - Arabidopsis
KW - purine catabolism
KW - senescence
KW - ureide
KW - UV-C
KW - wound
KW - xanthine dehydrogenase
KW - XDH
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U2 - 10.1093/jxb/erab441
DO - 10.1093/jxb/erab441
M3 - Article
AN - SCOPUS:85130482086
SN - 0022-0957
VL - 73
SP - 1016
EP - 1032
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 3
ER -