Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

S. B. Ryzhikov; A. N. Tikhonov

Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

Research output: Contribution to journal › Article › peer-review

Abstract

The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH₄Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700⁺ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

Original language	English
Pages (from-to)	67-70
Number of pages	4
Journal	Biophysics
Volume	36
Issue number	1
Publication status	Published - Dec 1 1991
Externally published	Yes

ASJC Scopus subject areas

Biophysics

Fingerprint Dive into the research topics of 'Use of water-soluble spin labels for measuring transmembrane pH difference in leaves'. Together they form a unique fingerprint.

Cite this

@article{91a0879d838a4e489b38b0d2a5dcf790,

title = "Use of water-soluble spin labels for measuring transmembrane pH difference in leaves",

abstract = "The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.",

author = "Ryzhikov, {S. B.} and Tikhonov, {A. N.}",

year = "1991",

month = dec,

day = "1",

language = "English",

volume = "36",

pages = "67--70",

journal = "Biophysics (Russian Federation)",

issn = "0006-3509",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "1",

}

TY - JOUR

T1 - Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

AU - Ryzhikov, S. B.

AU - Tikhonov, A. N.

PY - 1991/12/1

Y1 - 1991/12/1

N2 - The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

AB - The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

UR - http://www.scopus.com/inward/record.url?scp=44949271187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949271187&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:44949271187

VL - 36

SP - 67

EP - 70

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

SN - 0006-3509

IS - 1

ER -