Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

S. B. Ryzhikov, A. N. Tikhonov

Research output: Contribution to journalArticlepeer-review

Abstract

The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalBiophysics
Volume36
Issue number1
Publication statusPublished - Dec 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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