Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

S. B. Ryzhikov; A. N. Tikhonov

Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

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Abstract

The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH₄Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700⁺ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

Original language	English
Pages (from-to)	67-70
Number of pages	4
Journal	Biophysics
Volume	36
Issue number	1
Publication status	Published - Dec 1 1991
Externally published	Yes

ASJC Scopus subject areas

Biophysics

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abstract = "The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.",

author = "Ryzhikov, {S. B.} and Tikhonov, {A. N.}",

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T1 - Use of water-soluble spin labels for measuring transmembrane pH difference in leaves

AU - Ryzhikov, S. B.

AU - Tikhonov, A. N.

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N2 - The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

AB - The kinetics of photo-induced changes in the e.s.r. signal from the spin labels TEMPOamine and TEMPOcholine in the leaves of higher plants has been studied. Complex multiphasic kinetics of fall in the e.s.r. signal on exposure to white light was found. It is shown that the kinetics of this process is not sensitive to infiltration into the leaf of methyl viologen or NH4Cl but depends on addition of DCMU or FCCP. From the results coupled with those of an earlier kinetic study of the redox conversions of P700+ it is concluded that in the course of functioning of the chloroplasts in native conditions (in the leaf) the transmembrane pH difference in the thylacoids does not exceed ΔpH = 1 · 5.

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