Using nuclear targeting signals to enhance non-viral gene transfer

Chee Kai Chan, David A. Jans

Research output: Contribution to journalReview article

74 Citations (Scopus)

Abstract

Gene therapy involves the introduction of DNA-encoding therapeutic gene products into appropriate cells of an affected individual. The limitations of the approach relate largely to the poor efficiency of the delivery of the therapeutic DNA to the nucleus. This review examines recent work in the area of non-viral gene transfer, building on developments in the field of nuclear protein import and their application in the field of non-viral gene transfer. In particular, advances in the area of enhancing DNA targeting to the nucleus are discussed, including the use of modular nuclear targeting signals recognised by the cellular nuclear import machinery and DNA condensing agents to facilitate passage through the nuclear pore. Optimising nuclear DNA delivery through these and other strategies should assist greatly in rendering gene therapy a viable and realistic possibility for treating disease.

Original languageEnglish
Pages (from-to)119-130
Number of pages12
JournalImmunology and Cell Biology
Volume80
Issue number2
DOIs
Publication statusPublished - Apr 22 2002

Keywords

  • Atomic force microscopy
  • Gene therapy
  • Importin
  • Nuclear localization sequence
  • Nuclear targeting signal

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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